Amoeba Culture (Acanthamoeba, Naegleria)
| Sunquest Code: | PCLT | ||
| Epic Code: | LAB3235 | Epic Name: | Parasite Culture |
| Synonyms: | Cerebrospinal Fluid, Amoebas or Parasites; Naegleria; Parasites in Cerebrospinal Fluid; Acanthamoeba; Parasites in Tissue | ||
| Methodology: | Culture | ||
| CPT Code: | 87081 | ||
| Test Includes: | Culture for Acanthamoeba or Naegleria. | ||
| Turnaround Time: | Performed Mon-Fri, 0730-1530; results are reported within 10-12 days. | ||
| Special Instructions: |
Further questions regarding Naegleria fowleri should be directed to the IDDL Medical Director on-call. Call IDDL at 612-273-3665 for on-call information. Also see Amoeba Smears.
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Collection Instructions
| Specimen: | CSF, corneal scrapings, contact lens or lens solution, and brain or other tissues |
| Collection Instructions: |
· CSF: Obtain aseptically and submit 0.5-1 mL in sterile tube. · Corneal scraping: Collect in sterile vial with 0.5 mL amoeba saline obtained from laboratory. (0.5 mL sterile saline acceptable.) · Contact lens or solution: Send to lab in lens case or bottle, as is. Deliver to laboratory immediately.
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Processing and Shipping
| Specimen Processing: | Store at room temperature. |
| Shipping Instructions: | Ship at room temperature. Must arrive within 24 hours. |
| Test Performed at or Referral Lab | Infectious Diseases Diagnostic Lab-Microbiology (UMMC-East Bank) |
Interpretive
| Reference Range: | Negative. |
| Use: | Detect primary amebic meningoencephalitis, Acanthamoeba keratitis. |
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