Lactate, Grand Itasca

Sunquest Code:LACT  
Epic Code:LAB95Epic Name:Lactic Acid
Synonyms:Lactic Acid
Methodology:Bichromatic end point
CPT Code:83605
Turnaround Time:Performed and reported 24 hours/day.
Special Instructions:All other sites see Lactate Whole Blood (LACTW)

Collection Instructions

Optimal Volume:2.5 mL
Minimum\Peds Volume:0.6 mL
Container:Green (lithium heparin) on ICE (Gray (sodium fluoride + potassium oxalate))
Collection Instructions:Collect without the use of a tourniquet or within 3 minutes of applying the tourniquet, but before releasing the tourniquet. Do not allow patient to clench/unclench hand; this builds up lactic acid. Deliver to laboratory immediately. Samples on ice must be received and assayed within 60 minutes of collection. Room temperature samples must be received and assayed within 15 minutes of collection.
Causes for Rejection:Grossly lipemic or icteric specimens. Refrigerated samples greater than 24 hours old.
Contraindications:Lack of acidosis is not a contraindication for this test.

Processing and Shipping

Specimen Processing:Process immediately, separate within 15 minutes (grey top tube). Refrigerate up to 24 hours. Centrifuge at refrigerated temperature. Aliquot 1.25 mL, 0.2 mL minimum.
Shipping Instructions:Ship on dry ice.
Stability:Spun sample is stable at 2-8°C for 24 hours. Keep container stoppered. Heparinized plasma is acceptable; however, the following precautions must be rigidly adhered to in order to retard glycolysis: 1) collect on ice; 2) transport immediately; 3) centrifuge within 15 minutes of collection to avoid metabolism of glucose to lactate; 4) separate the plasma immediately and store on ice until analysis.
Test Performed at or Referral Lab Chemistry  (Grand Itasca GICH)


Critical Range:Greater than 2.5 mmol/L
Reference Range:0.5 - 2.2 mmol/L
Use:Identify lactic acidosis which characteristically presents as an increased anion gap metabolic acidosis.

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