1,25 Dihydroxy Vitamin D, Adult - Preferred for evaluating hypercalcemia or renal failure


Sunquest Code:VIT125  
Epic Code:LAB6615Epic Name:1,25 Dihydroxy Vitamin D
Synonyms:1, 25 Dihydroxycholecalciferol, Vitamin D
Methodology:Chemiluminescent immunoassay
CPT Code:82652
Turnaround Time:Testing is performed 2 times per week. Results are reported 3-7 days.
Special Instructions:Verify with the physician whether they are diagnosing Vitamin D deficiency or evaluating the patient for hypercalcemia or renal failure. Order VITD (LAB535) for deficiency.


Collection Instructions

Specimen:Blood
Optimal Volume:1 mL
Minimum\Peds Volume:0.8 mL
Container:Red or gold (gel) (Green (lithium heparin, gel), Purple (EDTA), Red (no gel))
Causes for Rejection:Grossly hemolyzed, icteric or lipemic samples as well as samples containing particulate matter.


Processing and Shipping

Specimen Processing:Centrifuge and aliquot 1 mL, 0.3 mL minimum. Store at room temperature or refrigerated.
Shipping Instructions:Ship at refrigerated temperature.
Stability:Stability: Samples are stable for 1 week at room temperature, 2 weeks refrigerated and 6 months frozen.
Test Performed at or Referral Lab Specialty Core  (UMMC-East Bank)


Interpretive

Reference Range:Adult:  19.9 - 79.3 pg/mL

Pediatric reference ranges are not available; order 1,25 Dihydroxy Vitamin D to Mayo-Pediatric with same name for pediatric patients.
Use:Vitamin D testing is not covered by government payers for screening. This test does not need to be ordered in combination with Total 25-Hydroxyvitamin D. This test is indicated for patients with renal failure, unexplained hypercalcemia or hypercalciuria (suspected granulomatous disease or lymphoma), suspected genetic childhood rickets or suspected tumor-induced ostomalacia. The recommended test for diagnosing Vitamin D deficiency is Total 25-Hydroxyvitamin D.


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