5-Hydroxyindoleacetic Acid, Urine, Quantitative

Sunquest Code:U5HIAA  
Epic Code:LAB3800Epic Name:5 HIAA Quantitative Urine
Synonyms:5-HIAA, Quantitative; Serotonin Metabolite, Quantitative; Indoles
Methodology:High performance liquid chromatography/Tandem mass spectrometry
Turnaround Time:Specimens are sent to reference laboratory Mon-Sat; results are reported within 1-4 days.

This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.

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Urine Collection Procedure

Collection Instructions

Specimen:Urine, 24 hour collection, or random
Optimal Volume:Submit entire 24 hour collection; analysis requires 1 mL.
Container:3 L plastic jug (Plastic leakproof container, sterile)
Collection Instructions:Refrigerate 24 hour samples during collection.
Patient Preparation:Patients should abstain, if possible, from medications, over-the-counter drugs, and herbal remedies for at least 72 hours prior to the test. Foods rich in serotonin (avocados, bananas, eggplant, pineapple, plums, tomatoes, walnuts) and medications that may affect metabolism of serotonin must be avoided at least 72 hours before and during collection of urine of HIAA.
Causes for Rejection:Room temperature specimens; samples other than urine.

Processing and Shipping

Specimen Processing:Mix collection well before aliquot is taken. Aliquot 4 mL, 1.0 mL minimum. Write duration and total volume on label/request form.
Shipping Instructions:Ship at refrigerated temperature.
Stability:Stability: 1 week - refrigerated; 2 weeks - frozen. Random sample accepted.
Test Performed at or Referral Lab Lab Sendouts  (ARUP)
Referral Lab number:80420 (HIAA)


Reference Range:
0-15 mg/24 hours. The HIAA-to-creatinine ratio will not be reported whenever the urine collection is random or other than 24 hours, or the urine volume is less than 400 mL/24 hours. 0-14 mg/g crt. No reference interval is available for results reported in units of mg/L.   Note: Foods and medications associated with altered urinary HIAA results:
Decreased HIAA Increased HIAA
Aspirin, chlorpromazine (Thorazine), corticotropin, dihydroxyphenylacetic acid, ethanol, gentisic acid, homogentisic acid, hydrazine derivatives, imipramine (Tofranil), isocarboxazid (Marplan), keto acids, levodopa, MAO inhibitors, methenamine, methyldopa (Aldomet), perchlorperazine, phenothiazines (Compazine), promazine, promethazine (Mepergan). Acetaminophen, acetanilide, caffeine, coumaric acid, diazepam (Valium), ephedrine, fluorouracil, glycerol guaiacolate (Guaifensin), melphalan (Akeran), mephenesin, methamphetamine (Desoxyn), methocarbamol (Robaxin), naproxen, nicotine, phenacetin, phenmetrazine, phenobarbital, phentolamine, rauwolfia, reserpine.
Use:HIAA is excreted in large quantities by individuals with carcinoid tumors, particularly of ileal origin. The absence of HIAA in patients strongly suspect for a carcinoid tumor may also be due to intermittent HIAA secretion or to insignificant metabolism of serotonin. Repeat determinations may be useful in the former circumstance and, in the latter case, measurements of blood serotonin may be of value.

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