Viral Culture - Non-Respiratory


Sunquest Code:VCULTN  
Epic Code:LAB7009Epic Name:Viral Culture Non-Respiratory
Synonyms:VCULT - Adenovirus, CMV, Cytomegalovirus or Enterovirus
Methodology:Cell culture
CPT Code:87252, 87253
Test Includes:Identification is billed separately from culture. Viruses that can be isolated by culture include: adenovirus, cytomegalovirus, enterovirus, herpes simplex virus, and varicella-zoster virus.
Turnaround Time:Specimens are sent to reference laboratory Mon-Sat; results are reported within 3-14 days.
Special Instructions:Indicate specimen source on container.


Collection Instructions

Specimen:See collection instructions.
Container:Plastic leakproof container, sterile (Sterile tube, Viral Transport Media)
Collection Instructions:

Deliver to laboratory immediately. The specimens are stored at refrigerated temperature.:

·    Eye swab or scraping: Use culture swab to collect conjunctival material. Alternatively, transfer conjunctival scrapings (using fine sterile spatula) into a viral transport media container.

·    Feces: Transfer 1 mL, 0.5 mL minimum into a sterile, leakproof container.

·    Swabs: Dacron polyester swabs or rayon tipped swabs are recommended.  Place in 3 mL of viral transport media. Indicate specimen source on container.

·    Tissue or lesions: Use a fresh set of sterile instruments for each tissue. Place in 3 mL viral transport media (preferred). Alternately, place each in separate sterile, leakproof container.

·    Urine/body fluids or CSF: Transfer 3 mL; 0.5 mL minimum of specimen into a sterile, leakproof container. Indicate specimen source on container.

 

Note: Because CMV can be shed in urine for months after CMV infection, urine specimens are appropriate only in diagnosis of congenital CMV in neonates.

 

Causes for Rejection:Calcium alginate, eSwab, dry or wood swabs.


Processing and Shipping

Specimen Processing:Store in refrigerator.
Shipping Instructions:Ship at refrigerated temperature. Must arrive within 72 hours.
Test Performed at or Referral Lab Lab Sendouts  (ARUP)
Referral Lab number:2006498


Interpretive

Reference Range:Negative
Use:To establish viral infection and to isolate virus for susceptibility assay.


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