Calcium, Ionized, Serum/Plasma

Sunquest Code:ICA  
Epic Code:LAB54Epic Name:Calcium Ionized
Order Instructions:Order ICAW for whole blood and ICA for plasma/serum.
Synonyms:Ionized Calcium
Methodology:Ion selective electrode
CPT Code:82330
Turnaround Time:Performed and reported 24 hours/day.

Collection Instructions

Optimal Volume:1 mL
Minimum\Peds Volume:0.5 mL
Container:Green (lithium heparin) on ICE (Green (lithium heparin, gel), Red or gold (gel))
Collection Instructions:

·          Do not remove stopper.

·          Place sample on ice and deliver to laboratory for processing within 1 hour of collection.

·          Acceptable without ice if received within 30 minutes of collection.

·          If unprocessed samples cannot be delivered to laboratory within 1 hour of collection, a whole blood ionized calcium testing should be requested. Whole blood samples are stable on ice for 4 hours.


*See Comments section for all stability requirements.


Causes for Rejection:Sample exposed to air during collection or transport not analyzed within 1 hour of collection, specimen on ice not received within 1 hour of collection, room temperature specimen not processed within 30 minutes of collection.

Processing and Shipping

Specimen Processing:

Centrifuge with stopper in place within one hour of collection. Do not open the tube or split the sample. Store in refrigerator.


If collected in red top (plain) tube, allow sample to clot for 15 minutes before centrifuging; do not remove stopper.


Whole blood specimens collected on ice and maintained anaerobically may be analyzed within 4 hours using test code ICAW. SI Unit Conversion Factors: mg/dL x .25 = mmol/L. See ICAW.


Shipping Instructions:Ship unopened (anaerobically) at refrigerated temperature.
Test Performed at or Referral Lab Chemistry  (UMMC-East Bank, Fairview Lakes - FLMC, Fairview Ridges - FRH, Fairview Northland - FNMC, Fairview Southdale - FSH, UMMC/UMMCH-West Bank, UMMC NICU, M Health Clinic and Surgery Center)


Critical Range:0-11 mo: Less than 3.5 mg/dL and greater than 7.0 mg/dL; 1 y & older: Less than 3.0 mg/dL and greater than 7.0 mg/dL
Reference Range:0-11 mo: 5.1-6.3 mg/dL; 1 year and older: 4.4-5.2 mg/dL


1)       Suspect hypocalcemia or hypercalcemia.

2)       Monitor treatment for calcemic disorder.


Click HERE to Report test errors or omissions.
*If no email program is associated with this computer, please contact: for TestID: 1233"