Borrelia burgdorferi Antibodies, IgG and IgM by Immunoblot


Sunquest Code:LYMAGM  
Epic Code:LAB3598Epic Name:LymeCONF IGG and IGM by Immunoblot
Synonyms:Lyme Antibodies, IgG & IgM by Immunoblot
Methodology:Immunoblot
Test Includes:Immunoblot confirmation of IgG and IgM Lyme antibodies
Turnaround Time:Specimens are sent to reference laboratory Mon-Sat. Results are reported in 1-2 days.
Special Instructions:Per CDC guidelines, if ELISA test result is negative, Immunoblot should not be performed.


Collection Instructions

Specimen:Blood
Optimal Volume:2 mL blood
Minimum\Peds Volume:0.5 mL blood
Container:Red or gold (gel)
Causes for Rejection:CSF, plasma, severely lipemic, hemolyzed specimens.


Processing and Shipping

Specimen Processing:Blood: Separate serum from cells ASAP. Centrifuge and aliquot 1 mL, 0.15 mL minimum. Store in refrigerator.
Shipping Instructions:Ship at refrigerated temperature.
Stability:Stability: 48 hours at room temperature; 2 weeks refrigerated; 1 year frozen.
Test Performed at or Referral Lab Lab Sendouts  (ARUP)
Referral Lab number:50254


Interpretive

Reference Range:
 
IgG       For this assay, a positive result is reported when any five or more of the following ten bands are present: 18, 23, 28, 30, 39, 41, 45, 58, 66, or 93 kDa. All other banding patterns are reported as negative.
IgM      For this assay, a positive result is reported when any two or more of the following bands are present: 23, 39, or 41 kDa. All other banding patterns are reported as negative.
 
Use:
This test should be used for confirmation of an equivocal or positive Borrelia burgdorferi total antibodies IgG and/or IgM test performed on patients less than 4 weeks after appearance of erythema migrans.  


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