Leishmania Culture, Trypanosome Culture - LG2756


Sunquest Code:LSMISC  
Epic Code:LAB4909Epic Name:Send Outs Misc Test
Methodology:Culture for Leishmania, Trypanosomes
Turnaround Time:Specimens are sent to reference laboratory Mon-Thur; results are reported within 4 weeks.
Special Instructions:

Please contact Frank Steurer about all laboratory issues related to diagnosing leishmaniasis – such as questions about obtaining culture medium from CDC, collecting specimens for various types of testing, shipping specimens to CDC (including the address and forms to use) and getting test results. Each situation/case should be individualized, with expert consultation.

Contact information

Frank Steurer

CDC, Parasitic Diseases Branch

1600 Clifton Road, Mailstop D-64 (bldg 23, room 9-439)

Atlanta, GA 30329

phone: 404-718-4175

email: fsteurer@cdc.gov

fax: 404-718-4195

 



Collection Instructions

Specimen:Leishmania: bone marrow, cutaneous ulcer aspirates, tissue biopsies, and tissue fluid aspirates. Trypanosoma: Aspirates, tissue.
Collection Instructions:

If possible, request culture medium before obtaining tissue specimens. CDC can provide tubes of culture medium by overnight express mail. Keep the tubes refrigerated until they are used; bring to room temperature shortly before inoculation. Once inoculated, keep at room temperature and mail to CDC as soon as possible, by overnight mail (preferably for arrival within 24 hours of when the specimens were obtained). Do not ship cultures on cold packs or dry ice, but keep the cultures away from heat.

 

If culture medium was not obtained in advance, consult CDC to discuss the options below:

·         If possible, place the specimen in tube/vial that contains a sterile buffered medium (e.g., buffered saline, RPMI, Eagle’s growth, Schneider’s, Tobie’s) with a neutral pH (~7-7.4). Use enough fluid to cover the specimen. Ship at room temperature, if the specimen will arrive at CDC within 24-48 hours. Otherwise, consult CDC about whether to refrigerate and ship on a cold pack, to minimize the potential for overgrowth of skin flora. (Of note, leishmanial culture medium from CDC contains antimicrobial agents.)

·         If no buffered medium is available, place the specimen in a sterile tube/vial; refrigerate the specimen and ship it on a cold pack by overnight mail to CDC.

 

 



Processing and Shipping

Specimen Processing:Do not store.
Shipping Instructions:Ship at room temperature.
Test Performed at or Referral Lab Lab Sendouts  (Center for Disease Control)


Interpretive

Reference Range:Negative
Use:The use of culture methods for diagnosis of hemoflagellate infections is often necessary.


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