PML/RARA: Molecular Detection of Acute Promyelocytic Leukemia


Sunquest Code:PMLRARCoPath Code:PMLRAR
Epic Code:LAB4724Epic Name:Acute Promyelocytic Leukemia PML/RARA
Synonyms:Molecular Detection of t(15;17); APL; RAR alpha; PML
Methodology:Reverse transcription of mRNA, amplification of cDNA, DNA separation, result interpretation
CPT Code:----------CPTCODES HERE----------
Turnaround Time:10 days


Collection Instructions

Specimen:Blood or bone marrow
Optimal Volume:10 mL blood; 5 mL bone marrow
Minimum\Peds Volume:5 mL blood; 2 mL bone marrow
Container:Yellow (ACD, Solution A) tube available from laboratory (Purple (EDTA))
Collection Instructions:
  • Blood: Collect in yellow ACD (Solution A) tube.
  • Bone marrow aspirate: Aspirate ACD solution into syringe from open ACD tube; collect 5 mL bone marrow into syringe.
   
Causes for Rejection:Specimens received more than 3 days from date of collection, incorrect volume or tube type, clotted or frozen specimens. RNA not received on dry ice.
Shared samples will not be accepted unless sent to Molecular first. Contamination will occur on automated hematology analyzers.  DNA extracted at non-CLIA certified (or equivalent) lab.


Processing and Shipping

Specimen Processing:Store in refrigerator. Do not freeze. Specimens received after 1400 Friday through Sunday will not be processed until Monday.
Shipping Instructions:Ship at refrigerated temperature. Must arrive within 24 hours.
Test Performed at or Referral Lab Molecular Diagnostics  (UMMC-East Bank)


Interpretive

Reference Range:Results are reported as PML/RAR transcript "negative" or "positive". See interpretive report.
Use:

The diagnosis and detection of minimal residual disease of the PML/RARA chimeric transcript associated with acute promyelocytic leukemia.

 

 



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