Legionella pneumophila Antibodies (Types 1-6), IgG by IFA


Sunquest Code:LPAB  
Epic Code:LAB3590Epic Name:LEGIONELLA PNEUMOPHILA ABY TYPES 1-6 IGG
Methodology:Indirect fluorescent antibody
CPT Code:86713
Turnaround Time:Specimens are sent to reference laboratory Mon-Sat; results are reported in 1-4 days.
Special Instructions:Parallel testing is preferred. Label samples as acute or convalescent. Convalescent specimens must be received within 30 days from receipt of the acute specimens.


Collection Instructions

Specimen:Blood
Optimal Volume:2 mL
Minimum\Peds Volume:0.4 mL
Container:Red or gold (gel) (Red (no gel))
Causes for Rejection:Hemolyzed, severely lipemic; plasma


Processing and Shipping

Specimen Processing:Separate serum from cells ASAP. Centrifuge and aliquot 1 mL, 0.1 mL minimum. Store in refrigerator.
Shipping Instructions:Ship at refrigerated temperature.
Test Performed at or Referral Lab Lab Sendouts  (ARUP)
Referral Lab number:50365 (LEGAB)


Interpretive

Reference Range:

Less than 1:128

Negative: No significant level of Legionella pneumophila Type 1-6 IgG antibody detected.

 

1:128

Equivocal: Questionable presence of Legionella pneumophila Type 1-6 IgG antibody detected. Repeat testing in 10-14 days may be helpful.

 

1:256 or greater

Positive: Presence of IgG of Legionella pneumophila Type 1-6 detected, suggestive of current or past infection.

Seroconversion between acute and convalescent sera is considered strong evidence of recent infection. The best evidence for infection is a significant change on two appropriately timed specimens where both tests are done in the same laboratory at the same time. This assay may detect infection by any of the serotypes 1-6. The CDC and many state health laboratories recommend testing only for antibody to Legionella pneumophilia Type 1. For equivocal or positive IFA results, the CDC protocol suggests follow-up testing for Legionella pneumophilia antibody Type 1.

 



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