Bordetella pertussis and parapertussis PCR - Nasopharyngeal Swab

Sunquest Code:BPDNA  
Epic Code:LAB8077Epic Name:B. Pertussis/Parapertussis PCR-NP
Synonyms:Whooping Cough; Bordetella PCR
Methodology:Multiplex Real-Time PCR
Turnaround Time:Performed Mon, Wed and Fri; results are reported in 2-3 days.
Associated Links:

IDDL (Microbiology/Virology) Collection Container Guidelines, Nasopharyngeal Swab Collection Procedure

Collection Instructions

Specimen:Nasopharyngeal swab in Universal Transport Medium
Container:BD Universal Transport Media
Collection Instructions:See Associated Links for Nasopharyngeal Swab Collection.
Causes for Rejection:Calcium-alginate swabs (shown to inhibit PCR), dry or moistened cotton swabs, samples other than NP swabs.

Processing and Shipping

Specimen Processing:Store refrigerated or frozen.
Shipping Instructions:Ship specimens at refrigerated or frozen temperature.
Stability:7 days refrigerated or frozen.
Test Performed at or Referral Lab Infectious Diseases Diagnostic Laboratory  (UMMC-East Bank)


Reference Range:Negative for Bordetella pertussis/parapertussis.
Use:This assay is an in vitro diagnostic test for the qualitative detection and differentiation of Bordetella pertussis and Bordetella parapertussis nucleic acids from nasopharyngeal specimens from patients with signs and symptoms of Bordetella infection of the respiratory tract.

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