Bordetella pertussis and parapertussis PCR

Sunquest Code:BPPPCR  
Epic Code:LAB5848Epic Name:Bordetella Pert Parapert DNA PCR
Synonyms:Whooping Cough
Methodology:Multiplex Real-Time PCR
CPT Code:87801
Turnaround Time:Performed Mon, Wed and Fri; results are reported in 2-3 days.
Associated Links:

IDDL (Microbiology/Virology) Collection Container Guidelines, Nasopharyngeal Swab Collection Procedure

Collection Instructions

Specimen:Respiratory specimens including:
  • posterior nasopharyngeal swab (preferred)
  • bronchoalveolar lavage (BAL)
  • respiratory aspirate
  • sputum
Optimal Volume:
  • Posterior nasopharyngeal swab: flocked swab in 1 mL or 3 mL Universal Transport Medium container
  • BAL/Respiratory aspirate/Sputum: 2 mL (0.5 mL minimum)
Collection Instructions:
  • Posterior nasopharyngeal: Flocked swab in Universal Transport medium. See Associated Links for Nasalpharyngeal Swab Collection procedure.
  • Bronch/Respiratory Aspirate/Sputum: Sterile, plastic leakproof container.
Causes for Rejection:Calcium-alginate swabs (shown to inhibit PCR), dry or moistened cotton swabs, specimens not in proper container or from non-respiratory sources. ESwabs (have not been validated).

Processing and Shipping

Specimen Processing:Posterior nasopharyngeal swabs are stable refrigerated for 5 days.
BAL/Respiratory aspirates/Sputum) are stable refrigerated for 5 days.
Shipping Instructions:Ship specimens at refrigerated temperature.
Test Performed at or Referral Lab Infectious Diseases Diagnostic Lab-Microbiology  (UMMC-East Bank)


Reference Range:Negative for Bordetella pertussis/parapertussis.

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