Amylase Isoenzymes


Sunquest Code:AISO  
Epic Code:LAB3272Epic Name:Amylase Isoenzymes
Methodology:Enzymatic
Test Includes:Total amylase, pancreatic and salivary amylase
Turnaround Time:Specimens are sent to reference laboratory Mon-Sat; results are reported within 1 day.


Collection Instructions

Specimen:Blood
Optimal Volume:2 mL
Minimum\Peds Volume:1.2 mL
Container:Red or gold (gel) (Green (lithium heparin, gel), Green (sodium heparin, no gel), Red (no gel))
Causes for Rejection:Hemolysis


Processing and Shipping

Specimen Processing:Allow specimen to clot completely. Centrifuge and aliquot 1 mL, 0.5 mL minimum within 2 hours of collection. Store in freezer.
Shipping Instructions:Ship at refrigerated temperature.
Stability:Stability: After separation from cells, 1 week at room temperature; 1 month refrigerated or frozen.
Test Performed at or Referral Lab Lab Sendouts  (ARUP)
Referral Lab number:20804


Interpretive

Reference Range:

Reference Interval

Pancreatic amylase

0-5 mo: **

6-35 mo: 2-28 U/L

3-6 y: 8-34 U/L
7-17 y: 9-39 U/L
18 y & older: 12-52 U/L

Salivary amylase

0-17 mo: **

18 mo & older: 9-86 U/L

Total amylase

0-2 days: **

3-90 d: 0-30 U/L

3-6 mo: 7-40 U/L

7-8 mo: 7-57 U/L

9-11 mo: 11-70 U/L

12-17 mo: 11-79 U/L

18-35 mo: 19-92 U/L

3-4 y: 26-106 y
5-12 y: 30-119 U/L
13 y & older: 30-110 U/L

Note: salivary amylase is calculated as the difference between the total and pancreatic amylase.

** Reference range not provided by reference laboratory.

 

Use:Fractionation of amylase is sometimes used to distinguish acute pancreatitis (p-amylase) from the other conditions which are characterized predominantly by salivary (s-amylase) elevations. Acute non-pancreatic conditions, which may also elevate amylase levels, include: parotitis, peritonitis, small intestine obstruction, perforated peptic ulcer, rupture of a tubal pregnancy, contraction of the sphincter of Oddi following morphine administration and mesenteric thrombosis.


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