Entamoeba histolytica (amebiasis), Antibody, IgG


Sunquest Code:EHIST  
Epic Code:LAB3448Epic Name:E HISTOLYTICA ANTIBODY
Synonyms:Amebiasis Antibody; Ambiasis histolytica Antibody; E. histolytica Antibody
Methodology:Enzyme-linked immunosorbent assay
CPT Code:86753
Turnaround Time:Specimens are sent to reference laboratory Mon-Sat; results are reported in 1-5 days.
Special Instructions:Parallel testing is preferred. Label samples as acute or convalescent. Convalescent specimens must be received within 30 days from receipt of the acute specimens.


Collection Instructions

Specimen:Blood
Optimal Volume:2 mL
Minimum\Peds Volume:0.4 mL
Container:Red or gold (gel) (Red (no gel))
Causes for Rejection:Hemolyzed, lipemic or contaminated samples.


Processing and Shipping

Specimen Processing:Separate serum from cells ASAP. Centrifuge and aliquot 1 mL, 0.1 mL minimum. Samples are stable refrigerated for 2 weeks.
Shipping Instructions:Ship on dry ice.
Test Performed at or Referral Lab Lab Sendouts  (ARUP)
Referral Lab number:50070 (AMB)


Interpretive

Reference Range:

0.79 IV or less

Negative: No significant level of detectable
E. histolytica IgG antibody.

0.8-1.19 IV

Equivocal: Repeat testing in 10-14 days may be helpful.

1.20 IV or greater

Positive: IgG antibody to E. histolytica detected, which may indicate a current or recent infection.

 

IV: Index Value: Optical Density (OD) of the patient serum divided by the OD of the cut-off value.

Seroconversion between acute and convalescent sera is considered strong evidence of recent infection. The best evidence for infection is a significant change on two appropriately timed specimens where both tests are done in the same laboratory at the same time. Note: In the case of extra-intestinal complications, a positive antibody can indicate amebiasis, even though stool findings are negative

 



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