Hepatitis B DNA Quantitative; Real-Time PCR


Sunquest Code:HBQNT  
Epic Code:LAB6463Epic Name:Hepatitis B DNA Quantitative
Synonyms:HBV Viral Load; HBQNT; HEPB; Hep B
Methodology:Real-time polymerase chain reaction (PCR)
CPT Code:87517
Turnaround Time:Testing is performed twice weekly; results are reported within 4 days.


Collection Instructions

Specimen:Blood
Optimal Volume:4 mL
Minimum\Peds Volume:2.2 mL
Container:10 mL Purple EDTA tube (Red or gold (gel))
Causes for Rejection:Heparinized samples, frozen whole blood, specimen types other than EDTA plasma or red or gold (gel) serum are not acceptable for testing.


Processing and Shipping

Specimen Processing:Within 24 hours of collection, centrifuge and aliquot 2 mL, 1 mL minimum. Store refrigerated or frozen.
Shipping Instructions:Ship at refrigerated or frozen temperature.
Test Performed at or Referral Lab Infectious Diseases Diagnostic Laboratory-Virology  (UMMC-East Bank)


Interpretive

Reference Range:

Not detected. This assay has a result range of 20 to 170,000,000 IU/mL (1.3 to 8.23 log IU/mL).

 

A 'Not Detected' result indicates that the assay was unable to detect HBV DNA within the specimen.

 

A '<20 IU/mL (<1.3 log IU/mL)' result indicates that HBV DNA is detected, but the level present is less than the lower quantification limit of the assay.

 

A '>170,000,000 IU/mL (>8.23 log IU/mL)' result indicates that HBV DNA is detected, but the level present is above the upper quantification limit of the assay.

 

Use:

This is a nucleic acid amplification test for the quantification of hepatitis B virus DNA in serum or plasma. Uses include:

·          Diagnose some cases of early acute HBV infection (before the appearance of HBsAg).

·          Distinguish active from inactive HBV infection.

·          Monitor response to anti-HBV therapy.

·          Quantification of HBV DNA in plasma/serum of patients with chronic HBV infection.

 

 



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