Lyme Antibody, IgG by Immunoblot (Confirmation)


Sunquest Code:LYWBG  
Epic Code:LAB3601Epic Name:Lyme Confirm IGG by Immunoblot
Synonyms:Borrelia burgdorferi Antibodies, IgG by Western Blot
Methodology:Immunoblot
CPT Code:86617
Turnaround Time:Specimens are sent to reference laboratory Mon-Sat; results are reported in 1-3 days.


Collection Instructions

Specimen:Blood
Optimal Volume:2 mL
Minimum\Peds Volume:0.4 mL
Container:Red or gold (gel) (Red (no gel))
Causes for Rejection:Severely lipemic or hemolyzed specimens; CSF or plasma


Processing and Shipping

Specimen Processing:Separate serum from cells ASAP. Centrifuge and aliquot 1 mL, 0.1 mL minimum. Store in refrigerator.
Shipping Instructions:Ship at refrigerated temperature.
Test Performed at or Referral Lab Lab Sendouts  (ARUP)
Referral Lab number:50255


Interpretive

Reference Range:Negative. IgG Positive: Any 5 of the following 10 bands: 18, 23, 28, 30, 39, 41, 45, 58, 66, or 93 kDa. IgG Negative: Any pattern that does not meet the IgG positive criteria.
Use:This test should be used for confirmation of an equivocal or positive Borrelia burgdorferi Total Antibodies, IgG and or IgM test performed on patients greater than 4 weeks after disease onset. A negative result indicates that the Immunoblot evaluation for the Lyme antibody demonstrates no antibodies unique to Borrelia burgdorferi, and therefore is not supportive of Lyme disease. A positive result indicates that the Immnoblot evaluation for Borrelia burgdorferi antibody is consistent with the presence of antibody produced by patients in response to infection by Borrelia burgdorferi and suggests the presence of Lyme disease. Although the test has been shown to have a high degree of reliability for diagnostic purposes, laboratory data should always be correlated with clinical findings. Current CDC recommendations for the serological diagnosis of Lyme disease are to screen with a polyvalent ELISA test and confirm equivocals and positives with Immunoblot. Both IgM and IgG Immunoblots should be performed on samples obtained less than 4 weeks after appearance of erythema migrans. Only IgG Immunoblot is to be performed on samples greater than 4 weeks after disease onset. IgM Immunoblot in the chronic stage is not recommended and does not aid in the diagnosis of neuroborreliosis or chronic Lyme disease.


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