Rickettsia rickettsii Antibodies, IgG and IgM


Sunquest Code:RICAGM  
Epic Code:LAB3717Epic Name:Rickettsia rickettsii Antibodies, IgG & IgM
Synonyms:Rocky Mountain Spotted Fever Antibodies, IgG and IgM
Methodology:Indirect immunofluorescence antibody
CPT Code:----------CPTCODES HERE----------
Turnaround Time:Specimens are sent to reference laboratory Mon-Sat; results are reported in 1-3 days.
Special Instructions:Parallel testing is preferred. Label samples as acute or convalescent. Convalescent specimens must be received within 30 days from receipt of the acute specimens.


Collection Instructions

Specimen:Blood
Optimal Volume:2 mL
Minimum\Peds Volume:0.8 mL
Container:Red or gold (gel) (Red (no gel))
Causes for Rejection:Severely lipemic, hemolyzed.


Processing and Shipping

Specimen Processing:Separate serum from cells ASAP. Centrifuge and aliquot 1 mL, 0.3 mL minimum. Store in refrigerator.
Shipping Instructions:Ship at refrigerated temperature.
Test Performed at or Referral Lab Lab Sendouts  (ARUP)
Referral Lab number:50371


Interpretive

Reference Range:

Rickettsia rickettsii (Rocky Mountain Spotted Fever) Antibody, IgG

Less than 1:64

Negative: No significant level of Rickettsia rickettsii Antibody, IgG detected.

 

1:64-1:128

Low positive: Presence of Rickettsia rickettsii Antibody, IgG detected, suggestive of current or past infection.

 

1:256 or greater

Positive: Presence of Rickettsia rickettsii Antibody, IgG, suggestive of recent or current infection.

Rickettsia rickettsii (Rocky Mountain Spotted Fever) Antibody, IgM

Less than 1:64

Negative: No significant level of Rickettsia rickettsii Antibody, IgM detected.

 

1:64 or greater

Positive: Presence of Rickettsia rickettsii Antibody IgM detected, which may indicate current or recent infection; however, low levels of IgM antibodies may occasionally persist for more than 12 months post-infection.

 

 

Use:The best evidence for current infection is a significant change on two appropriately timed specimens, where both tests are done in the same laboratory at the same time. The CDC does not use IgM results for routine diagnostic testing of Rocky Mountain Spotted Fever, as the response may not be specific for the agent (resulting in false positives) and the IgM response may be persistent from past infection.


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