Lymphocyte Antigen Proliferation


Sunquest Code:LYMAGP  
Epic Code:LAB6030Epic Name:Lymphocyte Antigen Proliferation
Order Instructions:When ordered with Lymphocyte Mitogens Proliferation, cancel LYMAGP and LYMMP and order LAM to combine.
Synonyms:Lymphocyte Blastogenesis, Antigens
Methodology:Cell culture
CPT Code:86353 x2
Turnaround Time:Specimens are sent to reference laboratory Mon-Thur; results are reported in 9-10 days.
Compliance:

This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.



Collection Instructions

Specimen:Blood
Optimal Volume:10 mL and a 10 mL normal control from an unrelated donor
Minimum\Peds Volume:7 mL (adult) or 3 mL (pediatric) and a 7 mL normal control from an unrelated donor
Container:Green (sodium heparin, no gel) (Yellow (ACD, Solution A) tube available from laboratory)
Collection Instructions:Sample and sample must be collected Mon-Thur by noon. Specimen and control must be in the original tubes and arrive at the reference laboratory within 48 hours of collection time.Testing is performed Tue-Fri only.
Causes for Rejection:Refrigerated or frozen samples, or samples in transport longer than 48 hours. Samples collected in yellow (ACD Solution B)


Processing and Shipping

Specimen Processing:Whole blood. Do not process. Send sample and control at room temperature.
Shipping Instructions:Ship at room temperature. Must arrive within 48 hours. Ship to arrive Mon-Thur. Live lymphocytes required. Critical ambient; do not refrigerate or freeze.
Test Performed at or Referral Lab Lab Sendouts  (ARUP)
Referral Lab number:96055 (LAPP)


Interpretive

Reference Range:By report.
Use: Candida and tetanus antigens are tested independently in lymphocyte culture. Lymphocyte proliferation in response to these antigens is determined by 3H-thymidine incorporation. Results are reported as the counts per minute (CPM) antigen stimulated versus a control culture and a stimulation index (SI) which represents the ratio of mean CPM of the stimulated lymphocytes to the mean CPM of the unstimulated control.


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