Respiratory Virus Panel by PCR, Extended


Sunquest Code:RVPCR  
Epic Code:LAB6357Epic Name:Respiratory Virus Panel by PCR
Synonyms:Viral Panel, Respiratory; Extended Respiratory Virus Panel; VCRE; Influenza, RSV, Adeno, Parainfluenza, Rhinovirus, Metapneumovirus; Viral PCR
Methodology:Multiplex Real-time PCR
CPT Code:----------CPTCODES HERE----------
Test Includes:This test detects influenza A (H1 and H3) Influenza A 2009 H1N1, Influenza B, RSV A, RSV B, Parainfluenza (1, 2 and 3), Human Metapneumovirus, Human Rhinovirus, Adenovirus B/E and Adenovirus C.
Turnaround Time:Test is performed once per day, 7 days per week.
Special Instructions:Specimen source is required. Specimens are stable up to 7 days refrigerated after collection in universal viral transport media or up to 1 month if stored frozen.
Associated Links:

Nasal Wash Procedure, IDDL (Microbiology/Virology) Collection Container Guidelines, Nasopharyngeal Swab Collection



Collection Instructions

Specimen:Nasopharyngeal swab in universal viral transport media or 2 mL BAL/respiratory aspirate, 0.5 mL minimum
Container:BD Universal Transport Media (Plastic leakproof container, sterile)
Collection Instructions:
 
  • Bronchial wash: Obtain material in sterile container or add to tube of viral transport media.
  • Nasopharyngeal swab: Insert flocked swab into nostril to back of nasopahrynx. Rub nasopharyx and remove swab carefully. Put the swab into 1 mL viral transport media.
  • Nasal Wash: Using RSV sampling kit, wash nasopharynx with 2-3 mL sterile non-preserved saline. Immediately aspirate back and send wash material in syringe on ice ot laboratory.

For other respiratory samples, see Viral Culture, Respiratory (VCULTR).      
Causes for Rejection:Calcium-alginate swabs (shown to inhibit PCR), dry or moistened cotton swabs, sputum or non-respiratory specimens. Sputum, tracheal aspirates, tissue and throat swabs are not acceptable.


Processing and Shipping

Specimen Processing:Store in refrigerator.
Shipping Instructions:Ship at refrigerated temperature.
Test Performed at or Referral Lab Infectious Diseases Diagnostic Laboratory-Virology  (UMMC-East Bank)


Interpretive

Reference Range:No virus detected.
Use:The Respiratory Virus Panel is a qualitative, multiplex, diagnostic test for simultaneous detection and identification of multiple respiratory viral nucleic acids in individuals exhibiting signs and symptoms of respiratory infection. This test is appropriate for severely immunocompromised or critically ill patients.


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