Vitamin B1, Plasma - NOTE: Vitamin B1, Whole Blood is the preferred test


Sunquest Code:VITB1  
Epic Code:LAB3874Epic Name:Vitamin B1 Plasma
Order Instructions:Order this test ONLY if the physician order specifies a PLASMA Vitamin B1.
Methodology:High performance liquid chromatography
CPT Code:84425
Turnaround Time:Specimens are sent to reference laboratory Mon-Sat; results are reported in 2-4 days.
Special Instructions:Whole blood is the preferred specimen for thiamine assessment. Approximately 80% of thiamine present in whole blood is found in red blood cells. Refer to Vitamin B1 (Thiamine), Whole Blood. This assay does not distinguish thiamine from its phosphate esters.
Compliance:

This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.



Collection Instructions

Specimen:Blood
Optimal Volume:2 mL
Minimum\Peds Volume:0.6 mL
Container:Purple (EDTA) (Green (sodium heparin, no gel))
Causes for Rejection:Non-frozen samples; hemolyzed sample. Samples other than heparin or EDTA plasma


Processing and Shipping

Specimen Processing:Centrifuge and aliquot within 1 hour of collection, 1 mL, 0.2 mL minimum. Each vitamin level requested requires a separate aliquot tube. Store frozen.
Shipping Instructions:Ship on dry ice.
Test Performed at or Referral Lab Lab Sendouts  (ARUP)
Referral Lab number:80389


Interpretive

Reference Range:4-15 nmol/L. Thiamine (vitamin B1) is reported. However, thiamine diphosphate (TDP), the biologically active form of thiamine, is not found in measurable concentration in plasma, and is best determined in whole blood specimens. Plasma thiamine concentration reflects recent intake rather than body stores.
Use:Rarely indicated. Whole blood is the specimen of choice for thiamine assessment.


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