BCR-ABL1: Molecular Detection of Minor Breakpoint Region, p190, Quantitative


Sunquest Code:ALLQTCoPath Code:BCRMINPCR
Epic Code:LAB6579Epic Name:BCRminqt BCR-ABL minor breakpoint Quant ALL
Sunquest Code (non-Epic sites):MD  
Synonyms:Breakpoint Cluster Region (BCR) Analysis; Philadelphia Chromosome
Methodology:Reverse transcription of mRNA (RT-PCR), amplification of cDNA for quantitative detection of ALL (minor) type breakpoint; real time PCR.
CPT Code:----------CPTCODES HERE----------
Turnaround Time:Performed once per week; results are reported within 10 days.


Collection Instructions

Specimen:Blood or bone marrow. RNA.
Optimal Volume:10 mL blood; 3 mL bone marrow
Minimum\Peds Volume:5 mL blood; 1 mL bone marrow; 25 uL RNA at a concentration of 20 ng/uL.
Container:Yellow (ACD, Solution A) tube available from laboratory (Purple (EDTA))
Collection Instructions:
  • Blood: Collect in yellow ACD (Solution A) tube.
  • Bone marrow aspirate: Aspirate ACD (or EDTA) solution into syringe from open ACD (or EDTA) tube; collect 3 mL bone marrow into syringe.

Note: The use of an anticoagulant other than ACD (or EDTA) may interfere with the assay.  
Causes for Rejection:Blood or bone marrow specimen received more than 72 hours from the date of collection; incorrect volume or tube type; clotted or frozen specimens; RNA not received on dry ice. RNA extracted at non-CLIA certified (or equivalent) lab.
Shared samples will not be accepted unless sent to Molecular first. Contamination will occur on automated hematology analyzers.


Processing and Shipping

Specimen Processing:Store blood or bone marrow in refrigerator Do not freeze blood or bone marrow. Store RNA on dry ice.
Shipping Instructions:Ship blood or bone marrow at refrigerated temperature to arrive within 24 hour of collection, Mon-Fri only. See Cause for Rejection. Ship RNA on dry ice.
Test Performed at or Referral Lab Molecular Diagnostics  (UMMC-East Bank)


Interpretive

Reference Range:By report.
Use:

Monitor the tumor load, status of remission, the minimal residual disease and chronological expression level of the fusion transcripts for CML and AML patients.

 



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