Borrelia burgdorferi Antibodies, IgG and IgM by Immunoblot (CSF)


Sunquest Code:CLYMGM  
Epic Code:LAB3391Epic Name:Lyme IGG and IGM CSF by Immunoblot
Synonyms:Lyme Antibody Detection; Lymes Disease, CSF
Methodology:Immunoblot
Turnaround Time:Specimens are sent to reference laboratory Mon-Sat; results reported within 1-3 days.
Special Instructions:Parallel testing is preferred. Label samples as acute or convalescent.
Compliance:

This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however, FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.


Collection Instructions

Specimen:CSF
Optimal Volume:3 mL
Minimum\Peds Volume:2 mL
Container:Sterile tube
Causes for Rejection:Hemolyzed, contaminated or heat-inactivated specimens.


Processing and Shipping

Specimen Processing:Aliquot 3 mL, 2 mL minimum in an ARUP standard transport tube. Store in refrigerator.
Shipping Instructions:Ship at refrigerated temperature.
Stability:8 hours at room temperature; 2 weeks refrigerated; 1 year frozen.
Test Performed at or Referral Lab Lab Sendouts  (ARUP)
Referral Lab number:55260


Interpretive

Reference Range:

Negative;

IgG Positive

Any five of the following ten bands: 18, 23, 28, 30, 39, 41, 45, 58, 66, or 93 kDa.

IgG Negative

Any pattern that does not meet the IgG positive criteria.

IgM Positive

Any two of the following three bands: 23, 39, 41 kDa.

IgM Negative

Any pattern that does not meet the IgM positive criteria.

A negative result demonstrates no antibodies unique to Borrelia burgdorferi, and therefore is not supportive of Lyme disease.

A positive result is consistent with the presence of antibody produced by patients in response to infection by Borrelia burgdorferi and suggests presence of Lyme disease. Although the test has been shown to have a high degree of reliability for diagnostic purposes, laboratory data should be correlated with clinical findings.

The detection of antibodies to Borrelia burgdorferi in cerebrospinal fluid may indicate central nervous system infection. However, consideration must be given to possible contamination by blood or transfer of serum antibodies across the blood-brain barrier.

 

 

Use:Current CDC recommendations for the serologic diagnosis of Lyme Disease are to screen with polyvalent EIA test and confirm equivocal and positive results with Immunoblot. Both IgM and IgG Immunoblots should be performed on samples less than 4 weeks after appearance of erythema migrans. Only IgG Immunoblot should be performed on samples greater than 4 weeks after the disease onset. IgM Immunoblot in the chronic stage is not recommended and does not aid in the diagnosis of neuroborreliosis or chronic Lyme disease.


Click HERE to Report test errors or omissions.
*If no email program is associated with this computer, please contact:
systemlabguide@fairview.org for TestID: 3885"