Pyruvate (Special Collection Procedure)


Sunquest Code:PYRACD  
Epic Code:LAB744Epic Name:Pyruvic Acid
Synonyms:Pyruvic Acid
Methodology:Enzymatic
CPT Code:84210
Turnaround Time:Specimens are sent to Reference Laboratory Mon-Sat. Results reported in 1-2 days.


Collection Instructions

Specimen:Blood placed in special collection tube within 10 minutes of collection.
Optimal Volume:2 mL
Minimum\Peds Volume:1 mL
Collection Instructions:
Special handling required.
  • Collect 1-2 mL of blood into a green (sodium or lithium heparin) tube.
  • Immediately after blood is drawn, add exactly 1 mL whole blood to a chilled pyruvate collection tube containing 2 mL 8 percent (w/v) perchloric acid
  • Mix thoroughly for 30 seconds by inverting 10-15 times and place in ice bath for 10 minutes. Deliver to the lab immediately.
  • Processing must occur before the sample is transported between campuses. Immediately centrifuge mixed specimen at 1500 x g (RCF) for 10 minutes. Decant 2 mL supernatant to an ARUP Standard Transport Tube and freeze immediately (min 1 mL).
   
Patient Preparation:Patient should be fasting and at complete rest. Patient should avoid any exercise of the arm or hand before or during collection. Draw the specimen without the use of a tourniquet or within three minute of applying the tourniquet, but before releasing the tourniquet.
Causes for Rejection:Room temperature specimens. If less than 1 mL of blood is added to collection tube, pH of the supernatant will be too low for testing.


Processing and Shipping

Specimen Processing:Special handling required. Processing must occur before the sample is transported between campuses. Immediately centrifuge mixed specimen at 1500 x g (RCF) for 10 minutes. Decant 2 mL supernatant, 1 mL minimum, into an ARUP Standard Transport tube and freeze immediately. (min: 1 mL)
Shipping Instructions:Ship on dry ice.
Test Performed at or Referral Lab Lab Sendouts  (ARUP)
Referral Lab number:80310


Interpretive

Reference Range:0.030-0.107 mmol/L (venous blood)
Limitations:If less than 1 mL of blood is added to collection tube, pH of the supernatant will be too low for testing to be performed.
Use:Evaluate diabetic ketoacidosis and other metabolic disorders.


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