Vitamin D, 25-Hydroxy


Sunquest Code:VITD  
Epic Code:LAB535Epic Name:Vitamin D Deficiency
Synonyms:25-Hydroxycholecalciferol; 25-OH-Vitamin D Total; 25 Hydroxyergocalciferol; 25-Hydroxy Vitamin D; VITD; 25-OH Vitamin D Total (D2 and D3); 25-Hydroxyvitamin D2 and D3; Vitamin D Deficiency; Vitamin D Screening
Methodology:Chemiluminescent immunoassay
CPT Code:82306
Test Includes:25-OH vitamin D3 and 25-OH vitamin D2
Turnaround Time:Performed Mon-Fri; results are reported in 1-2 days.


Collection Instructions

Specimen:Blood
Optimal Volume:2 mL
Minimum\Peds Volume:0.6 mL
Container:Red or gold (gel) (Green (lithium heparin, gel), Green (sodium heparin, no gel), Purple (EDTA), Red (no gel))
Causes for Rejection:Gross hemolysis


Processing and Shipping

Specimen Processing:Centrifuge and leave sample in serum separator tube or aliquot 1 mL, 0.2 mL minimum within 2 hours of collection. Specimens collected in a green (lithium heparin or sodium heparin) tube must be centrifuged for at least five minutes before aliquoting or assaying sample.
Shipping Instructions:Ship at refrigerated temperature.
Test Performed at or Referral Lab Specialty Core  (UMMC-East Bank)


Interpretive

Reference Range:20-75 ug/L Season, race and dietary intake and treatment affect the concentration of 25-hydroxy-Vitamin D. Values may decrease during winter months and increase during summer months. Values 20-29 ug/L may indicate Vitamin D insufficiency and values less than 20 ug/L may indicate Vitamin D deficiency.
Use:Vitamin D testing is not covered by government payers for screening. This test does not need to be ordered in combination with 1,25 Dihydroxy Vitamin D. This test measures the combined total of both 25-OH vitamin D3 and 25-OH vitamin D2 and reports the combined total. Recovery to 25-OH Vitamin D2 is only about 80%. If an individual is on Vitamin D2 (ergocalciferol) supplementation, order VITD23 by LCMSMS (LAB585).
This test is indicated for patients with chronic kidney disease, cirrhosis, hypocalcemia, hypercalciuria, hypervitaminosis D, parathyroid disorders, malabsorption states, obstructive jaundice, osteomalacia, osteoporosis, osteosclerosis/petrosis, rickets and vitamin D deficiency on replacement therapy related to a condition listed previously, to monitor the efficacy of treatment.


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