Cytomegalovirus DNA by PCR, Quantitative, Plasma, Urine and Bronchial Lavage


Sunquest Code:CMQT  
Epic Code:LAB913Epic Name:CMV DNA Quantificaton
Synonyms:CMV Viral Load; CMV DNA by PCR; CMV PCR
Methodology:Real-time polymerase chain reaction (PCR)
Turnaround Time:Testing is performed Mon-Sat; results are reported within 2 days.
Special Instructions:Testing is performed on EDTA plasma (EDTAP or PL), urine (U) and bronchial lavage (BRO or BAL) only! Tissue specimens, amniotic fluid, CSF, pericardial fluid, bone marrow and pleural fluid have not been validated and are sent to a reference laboratory for testing.


Collection Instructions

Specimen:Plasma, bronchial lavage, urine
Optimal Volume:4 mL blood; 2 mL bronchial lavage or urine
Minimum\Peds Volume:2.2 mL blood (0.7 mL for children under 3 years of age or younger only); 1 mL bronchial lavage or urine
Container:10 mL Purple EDTA tube, Plastic leakproof container, sterile
Collection Instructions:A minimum plasma sample volume of 0.25 mL is acceptable for children 3 years of age or younger. Sample volumes of less than 1 mL plasma for patients greater than 3 years of age will be canceled as QNS.
Causes for Rejection:Specimens other than EDTA plasma, bronchial lavage or urine will be sent to reference laboratory for testing. Specimens that do not meet the minimum volume requirement will be rejected as quantity not sufficient. Urine specimens in grey top preservative tubes are not acceptable. Urine specimens collected on cotton balls are not accepted.


Processing and Shipping

Specimen Processing:
  • Whole blood: Centrifuge and aliquot 2 mL, 1 mL minimum plasma within 6 hours of collection. A minimum pediatric sample of 0.25 mL is acceptable for children 3 years of age or younger. This volume does not permit repeat analysis. Store in refrigerator or alternately freeze.
  • BAL or urine: Do not centrifuge bronchial or urine specimens. Aliquot 1 mL, 0.8 mL minimum. Write specimen type on aliquot tube.
Shipping Instructions:Ship plasma, BAL and urine at refrigerated or frozen temperature. Never freeze whole blood.
Stability:Plasma, BAL or urine are stable at refrigerated temperature (2-8 degrees C) up to 7 days or frozen (-20 to -80 degrees C) up to 40 days.
Test Performed at or Referral Lab Infectious Diseases Diagnostic Laboratory  (UMMC-East Bank)


Interpretive

Reference Range:Undetected. This assay has a result range of 137 to 9,100,000 IU/mL (2.1 to 7.0 log IU/mL). A 'Not Detected' result indicates that the assay was unable to detect CMV DNA within the specimen. A '137 IU/mL (<2.1 log IU/mL)' result indicates that CMV DNA is detected but the level present is less than the lower quantification limit of the assay. A '9,100,000 OI/mL >7.0 log IU/mL)' result indicates that CMVDNA is detected, but the level present is above the upper quantification limit of the assay. Pediatric samples of less than 1 mL will be released with the following statement: This sample was diluted 1:1 with a phosphate buffered saline solution to achieve the minimum volume for testing. Result reported should be interpreted with caution. Very low levels of CMV may not be detected.
Use:

This is a nucleic acid amplification test for the quantification of cytomegalovirus DNA in plasma, bronch lavage and urine.

·          Detection and quantification of cytomegalovirus (CMV) viremia.

·          Monitoring CMV disease progression and response to antiviral therapy.

 

 



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