Blastomyces dermatitidis Antigen EIA, Blood


Sunquest Code:BLASTB  
Epic Code:LAB6853Epic Name:Blastomyces AGN Quant EIA Blood
Methodology:Enzyme immunoassay
CPT Code:87449
Turnaround Time:Specimens are sent to reference laboratory Mon-Thur; results are reported within 3-4 days.
Special Instructions:To assess the course of antigen clearance in patients with positive results or with known blastomycosis, obtain follow-up specimens at least 14 days after the last prior specimen.


Collection Instructions

Specimen:Blood
Optimal Volume:4 mL
Minimum\Peds Volume:2.6 mL
Container:Red or gold (gel) (Green (lithium heparin, gel), Purple (EDTA), Red (no gel))
Causes for Rejection:Tissue, sputum, bronchial brushings, stool FNA, biopsy, tracheal or bone marrow aspirate, samples in transport media, fixative or isolator tubes. Samples too viscous to pipet.


Processing and Shipping

Specimen Processing:Centrifuge and aliquot 2 mL, 1.2 mL minimum. Store in refrigerator.
Shipping Instructions:Ship at refrigerated temperature.
Test Performed at or Referral Lab Lab Sendouts  (MiraVista Diagnostics)
Referral Lab number:316


Interpretive

Reference Range:None detected
Limitations:Heterophile antibodies and rheumatoid factor can cause positive interference. Cross reactions due to closely related antigenic epitopes are seen in patients with histoplasmosis, paracoccidiodomycosis, and pencilliosis. Rare cross reactions have been seen in patients with aspergillosis and cryptococcosis.
Use:Aid in the diagnosis of disseminated or acute pulmonary blastomycosis.


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