Neutrophil Oxidative Burst Assay (DHR)


Sunquest Code:NUTOXB  
Epic Code:LAB6035Epic Name:Neutrophil Oxidative Burst Assay (DHR)
Synonyms:Neutrophil NBT
Methodology:Flow Cytometry
CPT Code:86352
Turnaround Time:Specimens are sent to reference laboratory Mon-Sat; results are reported in 2-3 days.
Special Instructions:Patient and unrelated normal control samples must be collected at approximately the same time, but within 48 hours of test performance is acceptable.
Compliance:

This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.



Collection Instructions

Specimen:One 3 mL green (sodium heparin) AND one normal control in a 3 mL green (sodium heparin) from a healthily unrelated individual.
Optimal Volume:3 mL
Causes for Rejection:Specimens not sent in original collection container. Refrigerated or frozen samples or samples in transport longer than 48 hours.


Processing and Shipping

Specimen Processing:Whole blood. Do not process. Send in original container. Store at room temperature. Do not refrigerate or freeze.
Shipping Instructions:Ship at room temperature to arrive within 48 hours of collection.
Test Performed at or Referral Lab Lab Sendouts  (ARUP)
Referral Lab number:96657 (DHR)


Interpretive

Reference Range:By report. Interpretation comparing the patient results to the client normal control and the laboratory control will be provided by the medical director. If sample shows abnormal results when stimulated, and no control was sent, test should be resubmitted with control sample to validate the conditions of collection, processing and transport.
Use:White blood cells are incubated with dihydrorhodamine 123 (DHR) and catalase, then stimulated with Phorbol 12-Myristate 13-Acetate (PMA). Dihydrorhodamine oxidation to rhodamine by the respiratory burst or the cell is measured by flow cytometry. Results are reported as the ratio of the mean channel fluorescence of stimulated cells versus unstimulated cells, which yields a stimulation index (SI).


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