Enterovirus PCR on CSF


Sunquest Code:EVPCR  
Epic Code:LAB3456Epic Name:Enterovirus PCR CSF
Methodology:Reverse transcription/polymerase chain reaction (PCR)
CPT Code:----------CPTCODES HERE----------
Turnaround Time:Specimens are performed daily; results are reported in 24 hours.


Collection Instructions

Specimen:CSF
Optimal Volume:1 mL
Minimum\Peds Volume:0.5 mL
Container:Sterile tube (Microbiology containers)
Causes for Rejection:Specimens other than CSF.


Processing and Shipping

Specimen Processing:Aliquot 1 mL, 0.5 mL minimum. Refrigerate. Freeze if test will not be performed within 72 hours of collection. Centrifugation of the specimen is not recommended.
Shipping Instructions:Ship at refrigerated temperature to arrive within 72 hours. Alternatively, ship on dry ice.
Test Performed at or Referral Lab Infectious Diseases Diagnostic Lab-Microbiology  (UMMC-East Bank)


Interpretive

Critical Range:Positive
Reference Range:Negative for Enterovirus by PCR. FDA approved assay performed using Cepheid GeneXpert real-time PCR. This assay is a reverse transcription polymerase chain reaction (RT-PCR) for presumptive qualitative detection of enterovirus RNA in CSF of patients with signs and symptoms of meningitis or meningoencephalitis. It does not rule out other causes of meningitis, or other viruses. Enterovirus (EV) includes coxsackieviruses, echoviruses, polioviruses, and newer types designated as enteroviruses. This assay is designed to detect a consensus region of the EV genome 5' untranslated region (UTR) between nucleotides 452 and 596. Negative EV results do not exclude enterovirus as a cause of meningitis, but that enterovirus was not detected. Positive EV results do not exclude other causes of meningitis.
Use:When suspecting viral (aseptic) meningitis or meningoencephalitis in infants, children and adults. Peak seasons are summer and fall, but also off-season at times.


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