HLA Auto Crossmatch, Recipient


Sunquest Code:ATOXMR  
Epic Code:LAB6470Epic Name:HLA Auto Crossmatch
Order Instructions:PWB Transplant clinic delivers to Immunology on East Bank; ACL sends via 30 minute courier on West Bank. Call Immunology 612-273-3100 for additional minimum volume information.
Synonyms:Auto-Flow Crossmatch; T and B Lymphocyte, Flow Crossmatch
Methodology:Flow cytometry
CPT Code:----------CPTCODES HERE----------
Test Includes:Selected patient sera are titered and crossmatched against allogeneic and autologous T and B lymphocytes.
Turnaround Time:Results are reported within 5 days.
Special Instructions:Deliver directly to Immunology Laboratory (7-139 PWB) immediately after collection. If this test is being added on, comment ADD-ON in the Comment field when placing a normal order in Epic. Immunology will receive the printout directly. All other lab areas should discard the Immunology ADD-ON printout.


Collection Instructions

Specimen:Blood
Optimal Volume:14 mL red (plain) tube AND 40 mL Yellow (ACD, Solution A) tube
Minimum\Peds Volume:3 mL red (plain) tube AND 10 mL Yellow (ACD, Solution A) tube
Container:Red (no gel), Yellow (ACD, Solution A) tube available from laboratory (Red (gel))
Collection Instructions:Collect specimen prior to dialysis. Inform lab if patient is receiving Rituxamib.
Causes for Rejection:ACD: Clotted specimen.
Red: Gross hemolysis, immunosuppression, drug interference
Contraindications:Serum will not clot (if collected during or immediately after dialysis).


Processing and Shipping

Specimen Processing:Store at room temperature.
Shipping Instructions:Ship at room temperature. Testing must be performed within 3 days of collection. Do not send routine testing on Friday.
Test Performed at or Referral Lab Immunology  (UMMC-East Bank)


Interpretive

Use:Determine presence or absence in potential recipient's serum of HLA antibody reactive with potential donor's cells which may result in hyperacute rejection of a transplant.


Click HERE to Report test errors or omissions.
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