Culture, Amniotic Fluid/Chorionic Villi

Sunquest Code:ACVSCOCoPath Code:AMPROC
Epic Code:LAB4660Epic Name:Amnio/CVS, Culture Only
Sunquest Code (non-Epic sites):ACVSCO  
Methodology:Insitu cell culture only
Turnaround Time:Turnaround time varies with clinical situation and time required for adeuqate culture to grow, typically 10-14 days.
Special Instructions:Test request form requires:
  • Date and time of specimen collection
  • Specimen type
  • Patient information: date of birth, sex and medical record number
  • Orderin physician and contact number
  • Reason for referral
  • Clinical status
  • Information regarding medications or transfusions
  • Previous or pending genetic testing results
Associated Links:

Genetic Testing Consent Form

Collection Instructions

Specimen:Amniotic Fluid/Chorionic Villi
Optimal Volume:Amniotic Fluid: 20 mL
Chorionic Villi: 30 mg clean villi, free of decidua
Minimum\Peds Volume:Amniotic Fluid: 10 mL
Chorionic Villi: 10 mg clean villi, free of decidua
Container:Sterile tube
Collection Instructions:Maintain sterility during collection. Transport to the laboratory as soon as possible.
Collect CVS in tissue culture medium provided by the Cytogenetics laboratoyr; use sterile technique.

Processing and Shipping

Specimen Processing:Specimens must be received in the Cytogenetics Laboratory Mon-Fri by 5:30 pm; weekends and holidays by 4:30 pm. Specimens received after these cutoffs will be processed the following day. Store at room temperature.
Shipping Instructions:Ship at room temperature ASAP. For optimal testing results the specimen must arrive within 24 hours.
Test Performed at or Referral Lab Cytogenetics  (UMMC-East Bank)

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