Cytotoxic T-Lymphocyte


Sunquest Code:CTLF  
Epic Code:LAB5679Epic Name:Cytotoxic T-Lymphocyte
Synonyms:CTL Function; Cytotoxic T Lymphocyte
Methodology:Chromium release
Turnaround Time:Specimens are sent to reference laboratory Mon-Thur by 1500; results are reported within 14 days.
Special Instructions:A completed requisition and genetic consent form must accompany each sample. Samples must be received in testing laboratory within 24 hours of collection. Call 513-636-4685 with tracking numbers.
Associated Links:

Genetic Testing Consent Form, Customized Volumes by ALC



Collection Instructions

Specimen:Blood
Optimal Volume:10 mL
Container:Green (sodium heparin, no gel)
Collection Instructions:Specimens must be collected after 10:00 am  and received in East Bank Core Lab by 1400, Monday through Thursday. Do not collect the day before a holiday.

Volumes requested assume abnormal absolute lymphocyte count (ALC). If the ALC is abnormal, use the Customized Volumes by ALC information to obtain correct volume, See Associated Links.
Causes for Rejection:Samples received in testing laboratory greater than 24 hours old.


Processing and Shipping

Specimen Processing:Whole blood. Do not process.
Shipping Instructions:Ship whole blood in original container at room temperature. Samples must be received in testing laboratory within 24 hours of collection and before 11:00 am on Fridays. Protect from temperature extremes.
Stability:24 hours at room temperature.
Test Performed at or Referral Lab Lab Sendouts  (Cincinnati Children's Hospital Medical Center)
Referral Lab number:2900650


Interpretive

Reference Range:By report.
Use:Cytotoxic T lymphocytes (CTL) are a subset of lymphocytes that have the ability to lyse target cells bearing specific antigens. To assess the functionality of CTL's, target cells previously primed to contain radioactive chromium within the cell membrane, are incubated with patient or control lymphocytes. Lysis of the target cells by the CTL's is measured by the amount of chromium released into the supernatant.


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