Lymphocyte Antigen and Mitogen Proliferation - Collection Prior to Noon


Sunquest Code:LAM  
Epic Code:LAB3580Epic Name:Lymphocyte Agn and Mitogen PROLIF Panel
Synonyms:Mitogen and Antigen Stimulation
Methodology:Cell culture
CPT Code:86353 x5
Turnaround Time:Specimens are sent to reference laboratory Mon-Thurs; results are reported in 9-10 days.
Compliance:

This test was developed and its performance characteristics determined by ARUP Laboratories. The U.S. Food and Drug Administration has not approved or cleared this test; however FDA clearance or approval is not currently required for clinical use. The results are not intended to be used as the sole means for clinical diagnosis or patient management decisions.



Collection Instructions

Specimen:Blood
Optimal Volume:10 mL and a 10 mL normal control from an unrelated donor
Minimum\Peds Volume:7 mL (adult) or 3 mL (pediatric) and 7 mL normal control from an unrelated donor
Container:Yellow (ACD, Solution A) tube available from laboratory (Green (sodium heparin, no gel))
Collection Instructions:Sample and control must be collected Monday through Thursday by noon. Specimen and control must be in the original tubes and arrive at the reference laboratory within 48 hours of collection time. Testing is performed Tuesday through Friday only.
Causes for Rejection:Refrigerated or frozen samples, or samples in transport longer than 48 hours.


Processing and Shipping

Specimen Processing:Whole blood. Do not process. Send sample and control at room temperature.
Shipping Instructions:Ship at room temperature. Must arrive by noon Tuesday through Friday. Live lymphocytes required. Critical ambient, do not refrigerate or freeze.
Test Performed at or Referral Lab Lab Sendouts  (ARUP)
Referral Lab number:96056 (LAM)


Interpretive

Reference Range:By report.
Use:

Phytohemagglutinin, concanavalin A, pokeweed mitogen, Candida antigen, and tetanus antigen are tested independently in lymphocyte culture. Lymphocyte proliferation in response to these mitogens and antigens is determined by 3H-thymidine incorporation.

 

Results are reported as counts per minute (CPM) mitogen or antigen stimulated versus a control culture and a Simulation Index (SI) which represents the ratio of CPM of the stimulated lymphocytes to the mean CPM of the unstimulated control.

 



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