Lymphocyte Activation Markers


Sunquest Code:LYMACT  
Epic Code:LAB6076Epic Name:Lymphocyte Activation Markers
Methodology:Flow cytometry
CPT Code:88184, 88185 x8
Turnaround Time:Specimens are sent to reference laboratory Mon-Thur by 1500; results are reported within 2 days.
Special Instructions:A signed informed consent in the patient's medical record is required. The consent should not be sent to the laboratory. Samples must be received at testing site within 24 hour of collection. A completed requisition from must be sent with each sample. Please call 513-636-4685 with courier and sample tracking number. See Associated Links for both forms.
Associated Links:

Genetic Testing Consent Form, Cincinnati Children's Hospital Requisition Form



Collection Instructions

Specimen:Blood
Optimal Volume:2 mL
Minimum\Peds Volume:2 mL
Collection Instructions:Specimens must be collected after 10:00 am and received in East Bank Core Lab by 1400 Monday through Thursday. Do not collect the day before a holiday.
Causes for Rejection:Specimens greater than 24 hours old. Refrigerated or frozen specimens. Clotted, hemolyzed or centrifuged specimens.


Processing and Shipping

Specimen Processing:Whole blood. Do not process.
Shipping Instructions:Ship sample same at room temperature. Must arrive at testing site within 24 hours of collection and before 1700 on Fridays..
Test Performed at or Referral Lab Lab Sendouts  (Cincinnati Children's Hospital Medical Center)
Referral Lab number:2900100


Interpretive

Reference Range:By report.
Use:Measures of lymphocyte activation in the following areas: evaluation of cellular immune response, assessment of the activity of autoimmune disease processes. And monitoring of pre- and post-transplant for early signs of graft rejection, to name a few. Detection of activated T-cells is perceived as providing a more precise indication of the dynamics of immune function than could be obtained simply from counting absolute or relative numbers of different lymphocyte types in the blood. Quiescent lymphocytes, i.e. not motivated by an antigenic stimulus, express little if any surface appearance of the activation antigens (CD69, CD25, CD40L, CD134, CD95, CD71, HLA-DR) and only upon stimulation will these antigens up-regulate or over-express on the surface of lymphocytes.


Click HERE to Report test errors or omissions.
*If no email program is associated with this computer, please contact:
systemlabguide@fairview.org for TestID: 4864"