Bordetella pertussis Antibodies IgA and IgG by ELISA with Reflex to Immunoblot


Sunquest Code:BPTAGE  
Epic Code:LAB6759Epic Name:B Pertussis IGA IGG ELISA RFLX to IMMBLT
Synonyms:B Pertussis
Methodology:Enzyme-linked immunosorbent assay/Immunoblot
CPT Code:86615 x2, 86615
Turnaround Time:Specimens are sent to reference laboratory Mon-Sat; results are reported within 1-8 days.
Special Instructions:Parallel testing is preferred, and convalescent specimens must be received within 30 days from receipt of the acute specimens. Mark specimens plainly as "acute" or "convalescent".
Compliance:

Analyte Specific Reagent. This test was developed and its performance characteristics determined by ARUP Laboratories. The U. S. Food and Drug Administration has not approved or cleared this test; however, the FDA has determined that such clearance or approval is not necessary.



Collection Instructions

Specimen:Blood
Optimal Volume:2 mL
Minimum\Peds Volume:0.8 mL
Container:Red or gold (gel) (Red (no gel))
Causes for Rejection:Contaminated, heat-inactivated or severely lipemic specimens.


Processing and Shipping

Specimen Processing:Centrifuge and aliquot within 2 hours of collection, 1 mL, 0.3 mL minimum. Store in refrigerator.
Shipping Instructions:Ship at refrigerated temperature.
Test Performed at or Referral Lab Lab Sendouts  (ARUP)
Referral Lab number:2001774


Interpretive

Reference Range:
Bordatella pertussis Aby, IgA by ELISA:
0.9 IV or less Negative: No significant level of detectable B. pertussis IgA Aby
1.0-1.1 IV Equivocal: Repeat testing in 10-14 days may be helpful.
1.2 IV or greater      Positive: IgA antibody to B. pertussis detected, which may indicate a current or past exposure/immunization to  B. pertussis.

Bordetella pertussis Aby, IgA by Immunoblot: Negative

Bordetella pertussis Aby IgG by ELISA:
0.94 IV or less Negative: No signifcant level of detectal B. pertussis IgG antibody.
0.95-1.04 IV Equivocal: Repeat testing in 10-14 dyas may be helpful.
1.05 IV or greater       Positive: IgG antibody to B. pertussis detected, which may indicate a current or recent exposure/immunization to B. pertussis.

Bordetella pertussis Aby, IgG by Immunoblot: by report.
Use:If Bordetella pertussis Antibody, IgA by ELISA is 1.2 IV or greater, than Bordetella pertussis IgA Immunoblot testing will be added.
If Bordetella pertussis Antibody, IgG by ELISA is 1.05 IV or greater, then Bordetella pertussis IgG Immunoblot testing will be added.
Additional charges apply.


Click HERE to Report test errors or omissions.
*If no email program is associated with this computer, please contact:
systemlabguide@fairview.org for TestID: 5342"