Anti-Nuclear Antibodies (ANA) with Reflex to ANA, HEp-2 Substrate, IgG by IFA - LG5372


Sunquest Code:ARMISC  
Epic Code:LAB4909Epic Name:Send Outs Misc Test
Methodology:Enzyme-linked immunosorbent assay reflex to indirect fluorescent assay (IFA)
Turnaround Time:Specimens are sent to reference laboratory Mon-Sat; results are reported within 1-3 days.
Special Instructions:Anti-Nuclear Antibodies (ANA), IgG by ELISA: ANA specimens are screened using enzyme-linked immunosorbent assay (ELISA). If ANA are detected by ELISA, then Antinuclear Antibody (ANA), HEp-2, IgG by IFA will be added. Additional charges apply.
ANA determined by indirect fluorescence assay (IFA) use HEp-2 substrate and IgG-specific conjugate at a screening dilution of 1:80. If positive, patterns reported include homogeneous, speckled, centromere, nucleolar, nuclear dots, or cytoplasmic. All positive results are reported with endpoint titers.


Collection Instructions

Specimen:Blood
Optimal Volume:1 mL
Minimum\Peds Volume:0.8 mL
Container:Red or gold (gel) (Red (no gel))
Causes for Rejection:Non-serum, heat-inactivated, grossly hemolyzed and severely lipemic specimens.


Processing and Shipping

Specimen Processing:Centrifuge and aliquot within 2 hours of collection 0.5 mL, 0.3 mL minimum. Store in refrigerator.
Shipping Instructions:Ship at refrigerated temperature.
Stability:After separation from cells: 48 hours at room temperature, 2 weeks refrigerated; 1 year frozen.
Test Performed at or Referral Lab Lab Sendouts  (ARUP)
Referral Lab number:50080


Interpretive

Reference Range:Anti-Nuclear Antibodies (ANA), IgG by ELISA: Not Detected.
Nuclear Antibody (ANA)HEp-2 by IFA, IgG: Less than 1:80
Use:Aids in initial diagnosis of connective tissue disease.


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