Urine Culture, Midstream


Sunquest Code:URC  
Epic Code:LAB239Epic Name:Urine Culture
Synonyms:URC
Methodology:Quantitative culture
CPT Code:----------CPTCODES HERE----------
Test Includes:Culture of aerobic flora for 1,000 to >100,000 organisms/mL and susceptibility testing. Gram stain if requested.
Turnaround Time:Performed daily; results are reported within 2 days.
Special Instructions:Specimens must be delivered to laboratory within 30 minutes of collection if not refrigerated or collected in vacutainer urine C&S transport kit.


Collection Instructions

Specimen:Urine, clean catch or midstream collection, urostomy collection
Optimal Volume:5-10 mL
Minimum\Peds Volume:0.2 mL (4.0 mL min if using vacutainer C&S)
Collection Instructions:

Obtain first morning specimen for best results (overnight incubation in bladder yields highest bacterial counts). Refrigerate specimen if not delivered to laboratory within 30 minutes.

Female:

1.  Wash hands with soap and water; dry with disposable paper towel.

2.  With one hand, patient or personnel should spread the labia and keep them apart for remainder of procedure.

3.  Using povidone-Iodine swab, wipe down one side from front to back; with fresh swab, wipe other side from front to back. With fresh swab, wipe center from front to back. Repeat as necessary to clean thoroughly.

4.  Wipe and dry area with sterile gauze.

5.  Void small amount of urine into toilet or bedpan. Stop flow for a minute then continue flow into sterile container. Be careful not to touch inner part of container or lid as container is being capped.

Male:

1.  Wash hands with soap and water; dry with disposable paper towel.

2.  Retract foreskin if necessary.

3.  Using povidone/iodine swabs, clean glans and urethral opening thoroughly.

4.  Wipe and dry area with sterile gauze.

5.  Follow step 5 above.

Infants and Small Children:

1.  After cleaning appropriately, as above, place sterile urine bag over labia or penis.

2.  After 30 minutes, observe for the presence of urine. If no urine is present, re-clean patient and attach new bag.

3.  If impossible to obtain urine or if culture results yield a mixture of organisms, a suprapubic aspiration is recommended.

 

Contraindications:Do not culture urine collected from bedpan, urinal, or brought from patient's home.


Processing and Shipping

Specimen Processing:Store in refrigerator. Specimen must arrive within 24 hours. Vacutainer: store at room temperature. Must arrive within 48 hours.
Shipping Instructions:Ship at refrigerated temperature. Must arrive within 24 hours. Vacutainer: ship at room temperature. Must arrive within 48 hours.
Test Performed at or Referral Lab Infectious Diseases Diagnostic Lab-Microbiology  (Fairview Range - FRMC, UMMC-East Bank, Grand Itasca GICH)


Interpretive

Reference Range:No growth, normal urogenital flora.
Use:Isolate and identify potentially pathogenic organisms causing urinary tract infection.


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