Mumps Real-Time Polymerase Chain Reaction (RT-PCR)


Sunquest Code:MUMPCR  
Epic Code:LAB6862Epic Name:Mumps Confirmation PCR
Methodology:Real-time polymerase chain reaction (PCR)
Special Instructions:All suspect cases of mumps and specimens submitted for testing must be coordinated with an MDH epidemiologist at 651-201-5414 or toll-free at 1-877-676-5414.
Associated Links:

MDH Submission Form



Collection Instructions

Specimen:Order of preference: Buccal swab - Collect on 0-5 day of swelling, throat swab is a second choice - Collect a buccal swab and a urine sample on Day 6-9.
Optimal Volume:See Collection Instructions
Minimum\Peds Volume:See Collection Instructions
Container:See Collection Instructions (Plastic leakproof container, sterile)
Collection Instructions:Buccal swab: Massage the buccal cavity (the space near the upper rear molars between the cheek and the teeth) for 30 seconds and swab the area. Obtain a generous amount of saliva. Place swab in a sterile tube containing 2-3 mL of viral transport medium.
Throat swab:  (if unable to obtain a buccal specimen): Vigorously swab tonsillar areas and posterior nasopharynx and a viral culturette. Use tongue blade to depress tongue to prevent contamination of swab with saliva. Place swab into viral transport medium.
Urine specimen: Collect 10 mL of urine in a sterile urine container. First-morning voided specimens are ideal, but any urine collection is adequate. Have patient void directly into container; collect from the first part of the stream if possible.


Processing and Shipping

Specimen Processing:

 

·          Do not process. Store in refrigerator.

·          Send specimen with a completed MDH submission form.

·          Package the specimen utilizing category B shipping instructions.

·          Label the package for delivery to MDH and ship directly to MDH.

 

Shipping Instructions:Ship overnight at refrigerated temperature. Enclose the MDH submission form with specimen.
Test Performed at or Referral Lab Lab Sendouts  (Minnesota Department of Health)


Click HERE to Report test errors or omissions.
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