Next Generation Sequencing Oncology Tumor Testing


Sunquest Code:NGSONCoPath Code:NGSO
Epic Code:LAB6497Epic Name:Next Generation Sequencing Oncology
Synonyms:BRAF; BRAF V600E; BRAFMD; CALR; Calreticulin; CEBPA; EGRF; ERBB2; GATA1; IDH1; IDH2; TP53; MPL; MET; MYD88; NPM1; NRAS; PDGFRA; PIK3cA; TP53; WT1; Lung Cancer Panel; Thyroid Cancer Panel; Colorectal Cancer Panel; GIST; Gastrointestinal Tumor Panel; Melanoma Panel; Glioma Panel; Solid Tumor Panel; Meyloproliferative Neoplasma Panel; MPL Panel; Acute Myeloid Leukemia Panel; AML Panel; NGS; Nucleophosmin; D816V; Asp816Val; CEBPAM
Methodology:Next generation sequencing
CPT Code:81210, 81235, 81245, 81310, 81403, 81403, 81403, 81403, 81403, 81272, 81311, 81314, 81405, 81405, 81219, 81479, 81479, 81479, 81479, 81479, 81275, G0452, 81276
Test Includes:

 

Genes in each panel:

·          Lung Panel – BRAF, EGFR, ERBB2, HRAS, JAK2, KRAS, MET, NRAS, PIK3CA

·          Thyroid Panel – BRAF, HRAS, KRAS, NRAS, PIK3CA

·          Colorectal Panel – BRAF, KRAS, HRAS, NRAS, PIK3CA

·          GIST Panel – BRAF, KIT, PDGFRA

·          Melanoma Panel – BRAF, HRAS, KIT, NRAS, PDGFRA

·          Glioma Panel – IDH1, IDH2, TP53

·          Extended Solid Tumor Panel – BRAF, EGFR, ERBB2, HRAS, IDH1, IDH2, JAK2, KIT, KRAS, MET, NRAS, PDGFRA, PIK3CA, TP53

·          Myeloproliferative (Short) Panel – CALR, JAK2, MPL

·          Myeloproliferative (Long) Panel – CALR, JAK2, MPL, BCR-ABL (p210) Quantitative (reported separately in EIPC), BCR-ABL (p190) Qualitative (reported separately in EPIC)

·          AML (Short) Panel – FLT3, NPM1, CEBPA (If blast count is less than 30% testing is cancelled.)

·          AML (Long) Panel – FLT3, IDH1, IDH2, KIT, KRAS, NRAS, NPM1, PDGFRA, TP53, WT1, CEBPA (If blast count is less than 30% testing is cancelled.)

 

Turnaround Time:Performed Mon-Fri; results are reported within 10-14 days
Special Instructions:Refer to requisition form for available tests. (See Associated Links) Non-EPIC sites print out requisitions(s) and check gene(s) or panel requested. Order testing in EPIC as panels or use the individual gene order and select requested genes. Fixed samples: Include block number for testing.
 
**You must look at the actual Epic order to determine which of the actions below are required. May be ordered on blood, bone marrow, or fixed tissue. Only ordered once on each sample.
If specimen requested is BLOOD: COLLECT SPECIMEN, ORM the NGSON in SQ GenLab, batch the specimen and send to UMMC.
If specimen requested is bone marrow: When marrow is received in the lab, ORM the NGSON in SQ GenLab, batch the specimen and send to UMMC.
If specimen requested is fixed tissue: SPECIMEN HAS PREVIOUSLY BEEN COLLECTED and Molecular Diagnostics lab will locate the specimen.
**COMPLETE STEPS 1 and 2 below:
1. ORM the NGSON in SQ GenLab
2. Track the specimen in SMART/Tracking/Specimen/Tracking
Change the "SPOT" to UMDIAG and scan the CID on the NGSON label.
 
Associated Links:

MDL Oncology Tumor Marker Request Form, NGSON Order Instructions



Collection Instructions

Specimen:Blood, bone marrow, fixed tissue; fresh tissue
Optimal Volume:10 mL blood; 3 mL bone marrow; Fixed tissue: 5 unstained slides (10 microns thick), 1 H&E (10 microns thick). Fresh tissue: 5 mm x 5 mm x 5 mm.
Minimum\Peds Volume:3 mL blood; 1 mL bone marrow; Fixed tissue: 4 unstained slides (10 microns thick), 1 H&E (10 microns thick). Fresh tissue: 3 mm x 3 mm x 3 mm.
Container:Yellow (ACD, Solution A) tube available from laboratory (Plastic leakproof container, sterile, Purple (EDTA))
Collection Instructions:Follow the NGSON Order Instructions prior to sample collection. See Associated Links.
Causes for Rejection:Whole blood or bone marrow: frozen or clotted specimen, incorrect anticoagulant, specimen more than 5 days old. Tissue: decalcified tissue.
Shared blood and bone marrow samples will not be accepted unless sent to Molecular first. Contamination will occur on automated hematology analyzers.


Processing and Shipping

Specimen Processing:Do not process. Store at room temperature.
Shipping Instructions:Ship at room temperature. Do not refrigerate or freeze.
Test Performed at or Referral Lab Molecular Diagnostics  (UMMC-East Bank)


Interpretive

Reference Range:See interpretive report.


Click HERE to Report test errors or omissions.
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