Enteric Pathogen and Virus Panel by NA


Sunquest Code:EPASS  
Epic Code:LAB6610Epic Name:Enteric Pathogen and Virus Panel by NA
Synonyms:Stool Pathogens; Shiga Toxin 1 & 2; Salmonella; Shigella; Campylobacter; Yersinia enterocolitica; Vibrio; Norovirus; Rotavirus; Stool Culture; Fecal; Feces; SSCE; EPASS
Methodology:Multiplex nucleic acid test
CPT Code:----------CPTCODES HERE----------
Test Includes:Salmonella, Shigella and Campylobacter, Shiga Toxin 1&2, Yersinia enterocolitica, Vibrio, Norovirus and Rotavirus. Culture for bacterial pathogens will be performed if PCR is invalid.
Turnaround Time:Performed daily; results are reported within 24 hours.
Associated Links:

IDDL Stool Collection Container Guidelines



Collection Instructions

Specimen:Feces
Optimal Volume:3 mL
Minimum\Peds Volume:0.5 mL
Collection Instructions:Feces: Collect in clean, dry bedpan or onto newspaper placed over toilet seat. Those portions of stool containing blood, pus or mucus are especially significant. Immediately place specimen in Cary-Blair transport media (Para-Pak C&S); place at 2-8 C until transport to the lab. Sample must be placed in Cary-Blair within 2 hours of collection.
Causes for Rejection:Samples received in sterile containers more than 2 hours from collection.Preserved stool not received at performing laboratory within 48 hours will be cancelled. Samples received on patients hospitalized for greater than 3 days will be cancelled without prior consultation with Infectious Diseases Diagnostic Laboratory.


Processing and Shipping

Specimen Processing:Sample must be placed in Cary-Blair within 2 hours of collection. Preserved specmen must arrive in IDDL (performing laboratory) within 48 hours of collection. If collected as Fecal Swab, receiving lab must transfer to ParaPak C&S container within 2 hours of collection. Store specimens at refrigerated temperature. 
Shipping Instructions:Ship specimens at refrigerated temperature. Must arrive within 48 hours.
Test Performed at or Referral Lab Infectious Diseases Diagnostic Lab-Microbiology  (UMMC-East Bank)


Interpretive

Reference Range:Negative for all targets.
Use:
Verigene Enteric Pathogens Nucleic Acid Test (EP) is a multiplexed, qualitative test for simultaneous detection and identification of common pathogenic enteric bacteria, viruses and genetic virulence markers from stool (in Cary-Blair preservative medium). EP utilizes reverse transcription, PCR and array hybridization to detect the following targets:
  • Campylobacter Group (composed of C.coli, C.jejuni and C.lari)
  • Salmonella species
  • Shigella species (including S.dysenteriae, S.boydii, S.sonnei and S.flexneri)
  • Vibrio Group (compsed of V.cholerae and V.parahaemolyticus)
  • Yersinia enterocolitica
  • Norovirus GI/GII
  • Rotavirus A
  • Shiga Toxin 1 & 2 genes (Shiga toxin producing E.coli typically harbors one or both genes)
 


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