India Ink Preparation


Sunquest Code:INK  
Epic Code:LAB3571Epic Name:India Ink Prep CSF
Synonyms:Cerebrospinal Fluid, Preparation for Encapsulated Yeast; Cryptococcus India Ink Preparation
Methodology:Direct examination of wet mount with black ink for contrast.
CPT Code:87210
Test Includes:Direct examination of CSF for encapsulated yeast.
Turnaround Time:Performed 24 hours/day; turnaround time is 30 minutes.
Special Instructions:Because the sensitivity of this test is low, a fungus culture should also be ordered. If suspicion of cryptococcal infection is high, a cryptoccal antigen should also be ordered.


Collection Instructions

Specimen:CSF
Optimal Volume:1 mL
Minimum\Peds Volume:0.5 mL
Contraindications:Very high levels of protein in CSF may cause autoagglutination of India Ink particles and interfere with examination of smear.


Processing and Shipping

Specimen Processing:Store at room temperature.
Shipping Instructions:Ship at room temperature. Must arrive within 24 hours.
Test Performed at or Referral Lab Infectious Diseases Diagnostic Lab-Microbiology  (UMMC-East Bank, Fairview Southdale - FSH)


Interpretive

Reference Range:No encapsulated yeast seen.
Use:Establish the presence of Cryptococcus. If encapsulated yeast are observed there is a high correlation with the presence of Cryptococcus neoformans, viable or non-viable forms.


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