Trichomonas Vaginalis DNA by PCR to HCMC - Special Request Only - LG6589


Sunquest Code:LSMISC  
Epic Code:LAB4909Epic Name:Send Outs Misc Test
Synonyms:BV; Bacterial Vaginosis; Wet Prep
Methodology:Real-time PCR
CPT Code:----------CPTCODES HERE----------
Turnaround Time:Results are reported in 24-72 hours, Mon-Fri.
Special Instructions:To be ordered as a special request by Center for Safe and Healthy Children Clinic (Explorer Clinic) and Pediatric ER.
When ordering in EPIC, include contact phone or fax number for reporting Stat results.
All others order Trichomonas Vaginalis DNA by PCR (TVPCR).


Collection Instructions

Specimen:Female or Male urine, vaginal/endocervical swab
Container:Aptima Yellow tube (Aptima Orange tube, Aptima Purple tube)
Collection Instructions:Urine - Female or Male First Catch Collection
Note: First catch urine must be transferred to an Aptima Urine Transport Reagent tube within 4 hours of collection.
  1. The patient should not have urinated for at least 1 hour prior to collecting the specimen. Female patients should not cleanse the labial area prior to specimen collection. Male patients should not cleanse the top of the penis prior to specimen collection.
  2. Patient should collect first 20-50 mL of the initial urine stream into a sterile, plastic, preservative-free container. Collection of larger volumes may result in specimen dilution that may reduce test sensitivity.
  3. Ensure the urine is well-mixed in the urine cup before transferring sample to the Aptima Transport tube.
  4. Transfer approxmiately 7 mL of urine into the transport tube. Urine must be filled to level within the window indicated on the tube provided in the kit within 4 hours of collection. Do not overfill.
  5. Replace the cap on the Aptima Transport tube, tighten securely and invert 3-4 times to mix.
  6. Label the transport tube with appropriate patient information.

Female Endocervial Specimens
Note: Samples must be collected using the flocked collection swab and transport tube in the Aptima Unisex Swab (purple) or Aptima Vaginal Self-Collect Swab (orange) collection kit.  
  1. Remove excess mucus from the cervical os and surrounding mucosa using the large, individually wrapped cleaning swab. Discard this large swab once cervical mucus has been removed.
  2. Insert the flocked speicmen collection swab (white shaft flocked swab in the package) into the endocervical canal.
  3. Gently rotate the swab for 10-30 seconds in the endocervical canal to ensure adequate sampling.
  4. Withdraw the swab carefully.
  5. While holding the swab in the same hand, unscrew the cap from the Aptima tube. (Do not spill tube contents. If the contents of the tube are spilled, then use a new collection kit.)
  6. Immediately place the swab in the transport reagent tube.
  7. Carefully break the swab shaft against the side of the tube and discard the top portion of the swab shaft; use care to avoid splashing of contents.
  8. Re-cap the swab transport reagent tube and tighten cap securely. Invert or gently shake the tube 3-4 times to elute material from this swab. Avoid foaming.
  9. Label the transport tube with the appropriate patient information.

Female Vaginal Self-Collect Specimens  
  1. Open the outer peelpack (which contains the two package kit), identify the larger cleaning swab and discard it.
  2. Open the package that contains the Aptima tube and individually wrapped collection swab. Open the collection swab wrapper and remove the swab taking care not to touch the tip or lay it down.
  3. Carefully insert the swab into the vagina about 2 inches past the opening of the vagina and gently rotate the swab to 10-30 seconds. Make sure the swab touches the walls of the vagina so that moisture is absorbed by the swab and then withdraw the swab.
  4. While holding the swab in the same hand, unscrew the cap from the Aptima tube. (Do not spill the contents. If the contents are spilled, then use a new collection kit.)
  5. Immediately place the swab in the transport reagent tube.
  6. Carefully break the swab shaft against the side of the tube and discard the top portion of the swab shaft; use care to avoid splashing of contents. Wash with soap and water if exposed.
  7. Re-cap the swab transport tube and tighten cap securely.
  8. Return the tube as instructed by lab staff or provider for appropriate patient labeling.
 
Causes for Rejection:Swabs submitted other than Aptima swabs provided; transport tubes containing a cleaning swab or more than 1 swab. Midstream or catheterized urine samples, urine in preservative, overfilled or under filled urine transport tubes.


Processing and Shipping

Specimen Processing:Urine: Must be transferred to an Aptima Urine transport reagent tube within 4 hours of collection.
Swabs: Must be collected usig the flocked collection swab and transport tube in the Aptima Unisex Swab or Aptima Vaginal Self-Collect Swab collection kit.
Shipping Instructions:Send at room temperature to East Bank Core Lab for Sendout processing and shipping.
East Bank Core Sendouts: Ship at room temperature with STAT OnTime Courier.
Test Performed at or Referral Lab Lab Sendouts  (Hennepin County Medical Center)
Referral Lab number:Urine-LAB0001365, Genital-LAB0001364


Interpretive

Reference Range:Negative


Click HERE to Report test errors or omissions.
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