Acute Myeloid Leukemia (AML) Focused NGS Panel, Blood or Bone Marrow


Sunquest Code:AMLFCB  
Epic Code:LAB8048Epic Name:AML Focused NGS Panel Blood BM
Synonyms:FLT3; NPM1; CEBPA; IDH2; IDH1
Methodology:Next generation sequencing
Test Includes:Next generation sequencing of FLT3, IDH1, IDH2, KIT, NPM1, NRAS, KRAS, CEBPA, TP53.  FLT3 testing (ITD, TKD) will be reported additionally as a separate report within 3-5 days.
Turnaround Time:Performed Mon-Fri; results are reported within 10-14 days.
Special Instructions:A signed informed consent in the patient's medical record is required; the consent should not be sent to the laboratory. The link to the Genetic Testing Consent Form is provided as a convenience for the providers and genetic counselors.
Bone Marrow Transplant Patients: If a patient is the receipient of an allogeneic transplant, this test must be done on a pre-transplant sample. Contact the Molecular Diagnostics lab to see if a pre-transplant sample is available.
Associated Links:

Gene Specific CPT Codes for Genetic Sequencing, NGS Genetic Testing Consent Form



Collection Instructions

Specimen:Blood, Bone marrow
Optimal Volume:10 mL blood; 3 mL bone marrow
Minimum\Peds Volume:3 mL blood, 1 mL bone marrow
Container:Yellow (ACD, Solution A) tube available from laboratory (Purple (EDTA))
Collection Instructions:Bone marrow clot acceptable for testing.
Causes for Rejection:Whole blood: frozen or clotted specimen, incorrect anticoagulant, specimen more than 5 days old. All testing will be cancelled if engraftment testing is 100% donor engrafted in consultation with clinician.

Shared blood or bone marrow samples will not be accepted unless sent to Molecular first. Contamination will occur on automated hematology analyzers. The diagnostic sample for hematology panels (not including MPN) requires at least 20% blasts to perform testing.  Follow-up samples for the hematology pnels will be tested for all gene(s) positive in the diagnostic sample and FLT3. Any genes testing negative in the diagnostic sample will be cancelled.


Processing and Shipping

Specimen Processing:Do not process. Store at room temperature.
Shipping Instructions:Ship at room temperature. Do not refrigerate or freeze.
Stability:Whole blood samples are stable for 5 days at room temperature. Do not refrigerate or freeze.
Test Performed at or Referral Lab Molecular Diagnostics  (UMMC-East Bank)


Interpretive

Reference Range:See interpretive report.
Use:Focused AML panel includes next genertion sequencing of FLT3, IDH1, IDH2, KIT, NPM1, NRAS, CEBPA, TP53.  FLT testing (ITD, TKD) will be reported additionally as a separate report within 3-5 days.
All testing will be cancelled if engraftment testing is 100% donor engrafted in consultation with clinician.
The diagnostic sample requires at least 20% blasts to perform testing. Follow-up samples for AML will be tested for the entire gene positive in the diagnostic samples and FLT3. Any genes testing negative in the diagnostic sample will be cancelled


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