Acute Myeloid Leukemia (AML) Expanded NGS Panel, Blood or Bone Marrow


Sunquest Code:AMLEXBCoPath Code:AMLEXB
Epic Code:LAB8046Epic Name:AML Expanded NGS Panel Blood BM
Synonyms:NPM1; CEBPA; IHD1; IDH2; FLT3
Methodology:Next generation sequencing
Test Includes:FLT3, IDH1, IDH2, KIT, NPM1, NRAS, KRAS, ASXL1, BCOR, CBL, DNMT3A, GATA1, KMT2A, PHF6, RARA, RUNX1, TET2, TP53 and CEBPA. FLT3, ITD and TKD testing will be reported additionally as a separate report within 3-5 days.
Turnaround Time:Performed Mon-Fri; results are reported within 10-14 days.
Special Instructions:A signed informed consent in the patient's medical record is required; the consent should not be sent to the laboratory. The link to the Genetic Testing Consent Form is provided as a convenience for the providers and genetic counselors.
Bone Marrow Transplant Patients: If a patient is the recipeint of an allogeneic transplant, this test must be done on a pre-transplant sample. Contact the Molecular Diagnostics lab to see if a pre-transplant sample is available.
Associated Links:

Gene Specific CPT Codes for Genetic Sequencing, NGS Genetic Testing Consent Form



Collection Instructions

Specimen:Blood, bone marrow
Optimal Volume:10 mL blood; 3 mL bone marrow
Minimum\Peds Volume:3 mL blood; 1 mL bone marrow
Container:Yellow (ACD, Solution A) tube available from laboratory (Purple (EDTA))
Collection Instructions:Bone marrow clot acceptable for testing.
Causes for Rejection:Whole blood: frozen or clotted specimens, incorrect anticoagulant, specimen more than 5 days old. All testing will be cancelled if engraftment testing is 100% donor engrafted in consultation with clinician.

Shared blood or bone marrow samples will not be accepted unless sent to Molecular first. Contamination will occur on automated hematology analyzers. The diagnostic sample for hematology panels (not including MPN) requires at least 20% blasts to perform testing.

Follow-up samples for the hematology panels will be tested for all the gene(s) positive in the diagnostic sample and FLT3. Any genes testing negative in the diagnostic sample will be cancelled.


Processing and Shipping

Specimen Processing:Do not process. Store at room temperature.
Shipping Instructions:Ship at room temperature. Do not refrigerate or freeze.
Stability:Whole blood samples are stable for 5 days at room temperature. Do not refrigerate or freeze.
Test Performed at or Referral Lab Molecular Diagnostics  (UMMC-East Bank)


Interpretive

Reference Range:See interpretive report.
Use:The expanded AML panel includes next generation sequencing of FLT3, IDH1, IDH2, KIT, NPM1, NRAS, KRAS, ASXL1, BCOR, CBL, DNMT3A, GATA1, KMT2A, PHF6, RARA, RUNX1, TET2, TP53 and CEBPA. FLT3, ITD and TKD testing will be reported additionally as a separate report with 3-5 days.
The diagnostic sample requires at least 20% blasts to perform testing. Follow-up samples for AML will be tested for all gene(s) positive in the diagnostic sample and FLT3. Any genes testing negative in the diagnostic sample will be cancelled.


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