Chronic Lymphocytic Leukemia Focused NGS Panel


Sunquest Code:CLLBLDCoPath Code:CLLBLD
Epic Code:LAB8067Epic Name:Chronic Lymphocytic Leukemia Focused NGS Panel
Synonyms:CLL; NOTCH1; SF3B1; TP53; BTK; ATM; MYD88
Methodology:Next generation sequencing
Turnaround Time:Performed Mon-Fri; results are reported within 10-14 days.


Collection Instructions

Specimen:Blood, bone marrow
Optimal Volume:10 mL blood, 3 mL bone marrow
Minimum\Peds Volume:3 mL blood; 1 mL bone marrow
Container:Yellow (ACD, Solution A) tube available from laboratory (Purple (EDTA))
Collection Instructions:Bone marrow clot acceptable for testing.
Causes for Rejection:Whole blood: frozen or clotted specimen, incorrect anticoagulant, specimen more than 5 days old. All testing will be cancelled if engraftment testing is 100% donor engrafted in consultation with clinician.
Shared blood or bone marrow samples will not be accepted unless sent to Molecular first. Contamination will occur on automated hematology analyzers.
The diagnostic sample requires at least 20% blasts to perform testing. Follow-up samples for the hematology panels will be tested for all positive gene(s) inthe diagnostic sample.


Processing and Shipping

Specimen Processing:Do not process. Store at room temperature.
Shipping Instructions:Ship at room temperature. Do not refrigerate or freeze.
Stability:Whole blood samples are stable for 5 days at room temperature. Do not refrigerate or freeze.
Test Performed at or Referral Lab Molecular Diagnostics  (UMMC-East Bank)


Interpretive

Reference Range:See interpretive report.
Use:Testing is used to diagnose and classify risk of Chronic Lymphocytic Leukemia. Genest tested: ATM, BTK, MYD88, NOTCH1, SF3B1, TP53. Mutations in ATM, NOTCH1, SF3B1 and TP53 are associated with a poor outcome. Different treatment regimens may be considered in patients with TP53 mutations or del(17p). Specific mutations in BTK predict resistance to certain therapies.


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