COG Flow Cytometry Bone Marrow


Sunquest Code:COGBM  
Epic Code:LAB8108Epic Name:COG Flow Cytometry Bone Marrow
Synonyms:MRD
Methodology:Multi-color immunofluorescence and flow cytometry
Turnaround Time:Performed Mon-Fri; results are reported within 1-3 days.
Special Instructions:Testing is performed Mon-Fri, 8 am - 5 pm and on specimens arriving in the Flow Cytometry lab on Saturday before noon, with results reported in 1-3 business days. No testing is performed on Sundays or holidays. Contact the IFC laboratory at 612-273-5248 for further information. If testing is needed outside of scheduled hours, contact the hematopathology fellow.


Collection Instructions

Specimen:Bone marrow
Optimal Volume:4 mL
Minimum\Peds Volume:2 mL
Container:Syringe, sterile, no needle (Green (sodium heparin, no gel), Purple (EDTA))
Collection Instructions:Collect in a sterile syringe with 0.5-1.0 mL of 1000 unit heparin per 5 mL of bone marrow to prevent clotting. Alternately collect in a sterile syringe and transfer immediately to a green (sodium heparin, no gel) or purple (EDTA) tube.
Causes for Rejection:Improper specimen collection or handling; clotted specimen; specimen received >48 hours after collection.


Processing and Shipping

Specimen Processing:Do not process. Store in refrigerator.
Shipping Instructions:Ship at room temperature.
Stability:48 hours at room temperature or refrigerated.
Test Performed at or Referral Lab Immunophenotyping and Flow Cytometry  (UMMC-East Bank)


Interpretive

Reference Range:By report.
Use:Use this test code for patients enrolled in COG B-ALL trials requiring decentralized flow MRD testing. This test code is for day 8 peripheral blood specimens. Please contact the Flow Cytometry laboratory at 612-273-5248 with questions.


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