Glioma Panel Focused NGS


Sunquest Code:GLIFOCCoPath Code:GLIFOCNGSO
Epic Code:LAB8114Epic Name:Glioma Panel Focused NGS
Synonyms:MGMT; Glioblastoma; GBM; Central Nervous System (CNS)
Methodology:Next generation sequencing
Test Includes:Select regions of IDH1, IDH2, TP53, PTEN and BRAF. MGMT methylation analysis is a separate assay.
Turnaround Time:Performed Mon-Fri; results are reported in 10-14 days.
Special Instructions:Fixed samples: Include block number for testing


Collection Instructions

Specimen:Fixed tissue slides
Optimal Volume:5 unstained slides (10 microns thick) and 1 H&E slide (10 microns thick ) fixed tissue.
Minimum\Peds Volume:5 unstained slides (10 microns thick) and 1 H&E slide (10 microns thick ) fixed tissue.
Container:Plastic leakproof container, sterile
Causes for Rejection:Tissue: Decalcified tissue; fixatives other than formalin used. Samples containing less than 10% tumor will not be tested.


Processing and Shipping

Specimen Processing:Do not process. Store at room temperature.
Shipping Instructions:Ship at room temperature. Do not refrigerate or freeze.
Stability:D not refrigerate or freeze.
Test Performed at or Referral Lab Molecular Diagnostics  (UMMC-East Bank)


Interpretive

Reference Range:See interpretive report.
Use:This panel is indicated in the evaluation of CNS gliomas to aid in diagnostic categorization, prognostic assessment, and therapeutic management. Please note that this panel does NOT assess for genomic rearrangements of BRAF gene (including BRAF-KIAA1549).


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