Cryptosporidium Giardia Immunoassay


Sunquest Code:CRYGI  
Epic Code:LAB8150Epic Name:Cryptosporidium/Giardia Immunoassay
Synonyms:Stool Antigen; CRYAG
Methodology:Immunochromatographic assay
Turnaround Time:Performed Mon-Fri; results are reported within 1-3 days.
Associated Links:

IDDL Stool Collection Container Guidelines



Collection Instructions

Specimen:Feces
Optimal Volume:5-10 mL of liquid feces, or 1-5 gm of formed feces
Container:10% Formalin Transport (Plastic leakproof container, sterile, Cary Blair Transport Media or C&S (Fecal swab for low volume stools only), See Collection Instructions)
Collection Instructions:Collect feces in clean, dry bedpan or onto newspaper over the toilet. Place 5-10 mL of liquid or semi-solid feces, or 5 grams of formed feces into 10% formalin transport.
Causes for Rejection:Specimens in any other preservative than indicated above. Colonic washes, aspirates or other diluted samples are not accepted. Samples received in sterile containers more than 2 hours from collection.
Contraindications:Stool samples contaminated by products with an oily or particulate base, i.e., mineral oil, barium, etc., could interfere with the test and are not recommended.


Processing and Shipping

Specimen Processing:Unpreserved solid, semi-solid or liquid samples should be transferred to 10% formalin within 2 hours of collection and can be stored at room temperature, refrigerated or frozen. Alternately, Cary-Blair or C&S samples can be stored refrigerated.
Shipping Instructions:Ship formalin samples at room temperature or refrigerated; ship Cary-Balir or C&S samples refrigerated.
Stability:Formalin: 2 months at room temperature; refrigerated or frozen.
Cary-Blair or C&S: 2 weeks refrigerated; 2 months frozen.
Unpreserved samples: Solid, semi-solid or liquid samples should be transferred to 10% formalin within 2 hours of collection.
Test Performed at or Referral Lab Infectious Diseases Diagnostic Laboratory  (UMMC-East Bank)


Interpretive

Reference Range:Negative for Cryptosporidium parvum antigen. Negative for Giardia lamblia antigen.
Limitations:Negative results can occur in samples containing low levels of antigen. Multiple specimens collected over several days can be tested for patients suspected of being positive for Giardia or Cryptosporidium.
Use:This test is a rapid immunoassay for the qualitative detection of Cryptosporidium parvum and Giardia lamblia specific antigens in aqueous extracts of human fecal specimens. It is intended to aid in the detection of suspected Cryptosporidium or Giardia infections.


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