Carbapenemase Gene Detection, PCR - Rectal Swab


Sunquest Code:CRRECT  
Epic Code:LAB8140Epic Name:Carbapenemase Gene Detection, PCR - Rectal Swab
Synonyms:Carbapenem Resistant PCR; CRE PCR; CRO PCR; Carba-R
Methodology:Multiplex Real Time RT-PCR
Test Includes:KPC, NDM, VIM, OXA-48 and IMP Genes
Turnaround Time:Test is performed 24 hours per day/ 7 days a week. Results are reported within 4 hours of receipt in the Infectious Diseases Diagnostic Laboratory.
Associated Links:

IDDL Collection Container Guidelines



Collection Instructions

Specimen:Rectal Swab
Container:Cepheid Specimen Collection Dual Swab Device
Collection Instructions:Dual rectal swab in Cepheid Specimen Collection container only.
Causes for Rejection:Specimen types other than dual rectal swabs in the Cepheid Specimen Collection  container are not acceptable for testing with this methodology. Wooden swabs or calcium alginate swabs are not acceptable. ESwabs are not acceptable. Highly soiled swabs are not acceptable.
Contraindications:
  • This assay will generate a negative IMP result when testing samples containing IMP-7, IMP-13, or IMP-14. False negative results may be encountered in rectal specimens with P. aeruginosa containing the blaVIM gene and with A. baumanii containing the blaIMP gene.
  • Interference may be observed with barium sulfate at >0.1% w/v and Pepto-Bismol at >0.01% w/v. In rectal samples containing the VIM target, interference may occur if fecal fat is present at a concentration of 0.25% w/v.
  • The performance of the Xpert Carba-R assay has not been evaluated with rectal swab specimens from pediatric patients.


Processing and Shipping

Specimen Processing:Store at room temperature.
Shipping Instructions:Ship at room temperature.
Stability:Dual rectal swabs collected using the Cepheid Specimen Collection Device can be stored up to 5 days at room temperature.
Test Performed at or Referral Lab Infectious Diseases Diagnostic Laboratory  (UMMC-East Bank)


Interpretive

Reference Range:Not detected.
Use:This assay is intended as an aid to infection control in the detection of carbapenem non-susceptible bacteria that colonize patients in healthcare settings. It is not intended to guide or monitor treatment for carbapenem non-susceptible bacterial infections or to determine infection from carbapenem non-susceptible bacteria.


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