Gentamicin


Sunquest Code:GENT  
Epic Code:LAB28Epic Name:Gentamicin Level
Methodology:Turbidimetric rate
CPT Code:80170
Turnaround Time:Performed and reported 24 hours/day.
Special Instructions:Comment last dose information on request form.


Collection Instructions

Specimen:Blood
Optimal Volume:2.5 mL
Minimum\Peds Volume:0.6 mL; pediatric patients only: 0.4 mL in green (lithium heparin, no gel)
Container:Red (no gel) (Green (lithium heparin, no gel))
Collection Instructions:Trough levels: Collect immediately prior to the next dose. Peak levels: collect 60 minutes after the end of IV infusion; 1-2 hours if decreased renal function.
Causes for Rejection:Processed >2 hours after collection and patient is receiving beta-lactam antibiotics structurally similar to gentamicin (e.g. netilmicin, sagamicin and sisomicin) or cephalosporin; collection in a gel tube


Processing and Shipping

Specimen Processing:Centrifuge and assay immediately. If assaying is delayed more than 2 hours, then aliquot and freeze the sample, 1.25 mL, 0.2 mL minimum (adult) 0.1 mL minimum (peds).
Shipping Instructions:Ship on dry ice.
Test Performed at or Referral Lab Chemistry  (Fairview Range - FRMC, UMMC-East Bank, Fairview Lakes - FLMC, Fairview Ridges - FRH, Fairview Northland - FNMC, Fairview Southdale - FSH, UMMC/UMMCH-West Bank)


Interpretive

Critical Range:Greater than 16 mg/L for traditional dosing; Greater than 35 mg/L for single daily dosing. Trough: greater than 2 mg/L
Therapeutic Range:Trough <1 mg/L; Peak: 5.0-12.0 mg/L, depending on dosage.
Use:Monitor therapeutic dosing. Half-life is 1.5-3 h (adult <30 y); 3-6 h (adult >30 y); 4-8 h (neonate).


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