CD34 and CD3 Absolute Count


Sunquest Code:343ABS  
Epic Code:LAB8000Epic Name:CD34 and CD3 Absolute Count
Synonyms:Progenitor Cell Assay; Stem Cell Assay; Hematopoietic Progenitor Stem Cell Assay
Methodology:Single platform multi-color immunofluorescence and flow cytometry using modified ISHAGE guidelines
CPT Code:----------CPTCODES HERE----------
Test Includes:Enumeration of hematopoietic progenitor stem cells using a combination of CD45/CD34 monoclonal antibodies. Viability of CD34 positive cells is also assessed.
Turnaround Time:24 hours
Special Instructions:Specimens are accepted Mon-Fri, 24 hours/day/ Testing is performed Mon-Fri, 8 am - 5 pm.  If testing is needed outside of scheduled hours, contact the Flow Cytometry lab. Provide diagnosis, age, collection date and time, specimen type, and any product information. Samples should be sent STAT between 8 am - 5 pm, Mon-Fri.
Compliance:

This test was developed and its performance characteristics determined by University of Minnesota Medical Center, Fairview Clinical Laboratories. It has not been cleared or approved by the US Food and Drug Administration. FDA does not require this test to go through premarket FDA review. This test is used for clinical purposes and should not be regarded as investigational or for research. This laboratory is certified under the Clinical Laboratory Improvement Amendments (CLIA) as qualified to perform high complexity clinical testing.



Collection Instructions

Specimen:Blood, peripheral blood progenitor cell collection, processed bone marrow.
Optimal Volume:3 mL blood; 1 mL peripheral blood progenitor cell collection; 10 x 10^6 processed bone marrow cells; 1 mL cord blood
Minimum\Peds Volume:1 mL blood; 0.5 mL peripheral blood progenitor cell collection; 0.5 mL cord blood
Collection Instructions:
Collect all specimens aseptically:
  • Blood: Collect in purple top (EDTA) tube; mix well.
  • Peripheral Blood Progenitor Cell Cellection: Collect in purple top (EDTA) tube; mix well.
  • Processed Bone Marrow: Place processed cells in sterile container.
  • Cord Blood: Place prepared cells in a sterile container
 
Causes for Rejection:Improper specimen collection or handling; clotted specimen; specimen received more than 24 hours after collection.


Processing and Shipping

Specimen Processing:Do not centrifuge. Store in refrigerator. Contact IFC Laboratory prior to sending specimen. See comments.
Shipping Instructions:Ship at room temperature. Must arrive within 24 hours.
Test Performed at or Referral Lab Immunophenotyping and Flow Cytometry  (UMMC-East Bank)


Interpretive

Reference Range:
   
Use:Flow cytometric evaluation of CD34+ cell level is necessary for measuring the hematopoietic progenitor cells in dose requirement protocols of stem cell transplantation. Quantification of CD34+ cells is also used during mobilization of hematopoietic cells and leukophoresis procedures. The total number of CD34+ cells collected during leukophoresis can be estimated.


Click HERE to Report test errors or omissions.
*If no email program is associated with this computer, please contact:
systemlabguide@fairview.org for TestID: 758"