Cytology, Fine Needle Aspirate


Sunquest Code:FNACTCoPath Code:FNA-EUS
Epic Code:LAB534Epic Name:Fine Needle Aspiration
Sunquest Code (non-Epic sites):COPATH  
Synonyms:Aspiration Biopsy Cytology; Fine Needle Aspirate; Needle Aspiration Cytology; Cytology, Pancreas
Methodology:Preparation, staining and microscopic evaluation
CPT Code:----------CPTCODES HERE----------
Test Includes:Preparation and microscopic evaluation; if special stains or immunoperoxidase stains are indicated they are ordered by the pathologist and performed by laboratory at additional charge. Aspiration by a pathologist will add professional fee. May include on site interpretation by a pathologist if requested.
Turnaround Time:Performed Mon-Fri, 0800-1500; results are reported within 1-3 days. If special stains or immunohistochemical procedures are necessary, additional time may be necessary.
Special Instructions:Provide pertinent clinical history. Also note if special stains for Pneumocystis jiroveii or fungus on lung aspirate are desired. If a liquid sample is obtained, such as from a cyst, see Cytology, Cyst Fluid for preparation instructions. Slides must have two unique identifiers: Full name and date of birth or medical record #.


Collection Instructions

Specimen:Fine needle aspirate
Collection Instructions:
  Fine needle aspirates may be treated by either of two methods, depending upon source and type of specimen:
Smear Preparation
  • Ultrasound guided (syringe technique)
  1. When the specimen is given in a needle, eject 1-2 small drops of aspirated material on two slides (half an inch from the frosted end of the slide) and the rest of the specimen into the molecular medium for thyroids, RPMI if lymphoma is suspected, or formalin for all other specimens.
  2. Place a smearing slide on top of the aspirated material perpendicular to the slide with the material. Do not press down on the slide. Pull the smearing slide to the end of the bottom slide and all the way off to smear the slide.
  3. One slide should be air-dried. The other slide should be fixed immediately in 100% alcohol or spray-fixed until the slide is visibly wet.
  4. Repeat for each pass.
  • EBUS/EUS guided
  1. Put the entire specimen into a formalin container.
  2. If suspicious for lymphoma, put one entire pass into RPMI; order Leukemia Lymphoma Evaluation test in Epic and send separately to Flow Cytometry.


Thyroid Fine Needle Aspiration Only:
  • Submission of sample directly into molecular panel preservative: Place patient ID label on molecular panel tube and identify them with nodule. Use separate molecular panel tubes for each nodule. Put all of the material from pass 2 into the molecular panel tube. While slides are being fixed, put any remaining material from the needle into the molecular panel tube.  Discard needle in Sharps container.
  • Submission of sample for Affirma testing: Place patient ID label on the Affirma panel tube and identify them with module. Use separate Affirma tubes for each nodule. Put all of the matieral from pass 2 into the Affirma tube. Discard needle in Sharps container.
  • Submission for cell block: If cell block is needed, put pass 2 into formalin container. While slides are being fixed, put any remaining material from the needle into the formalin container.
 
Causes for Rejection:Syringe with needle attached. No two patient identifiers.


Processing and Shipping

Specimen Processing:Store at room temperature. RPMI store in refrigerator.
Shipping Instructions:Ship slides at room temperature. Ship any unfixed fluid at refrigerated temperature.
Test Performed at or Referral Lab Cytology  (UMMC-East Bank, Fairview Ridges - FRH, Fairview Southdale - FSH, UMMC/UMMCH-West Bank)


Interpretive

Reference Range:Results reported as negative to positive for malignant cells or other abnormalities.
Use:Diagnosis of primary or metastatic malignant neoplasms; aid in the diagnosis of infections with fungi; parasites (such as Pneumocystis jiroveii) or some viruses.


Click HERE to Report test errors or omissions.
*If no email program is associated with this computer, please contact:
systemlabguide@fairview.org for TestID: 818"